鼻咽癌是广西区域性高发的头颈部恶性肿瘤之一。已有研究表明miR-126-VEGF的调控在多种肿瘤的发生、发展中起着重要的作用，但在鼻咽癌中鲜见报道。课题组从一系列新的苦参碱衍生物中，发现其中的衍生物YYJ18具有比苦参碱更强的抑制鼻咽癌细胞生长的作用，且发现其机制与ERK1/2、p38和Akt信号通路变化有关。为进一步研究苦参碱及苦参碱衍生物抗鼻咽癌细胞作用的靶点及其差异，通过在鼻咽癌细胞中引入外源性的miR-126，观察miR-126对VEGF-MAPK/Akt通路的调控作用；同时，探讨苦参碱及苦参碱衍生物是否通过干预miR-126-VEGF调控，影响下游MAPK/Akt信号通路，进而促进鼻咽癌细胞凋亡和抑制鼻咽癌细胞的增殖、侵袭转移。本项目为明确苦参碱衍生物具体作用机理，特别对靶基因发现及功能的研究提供实验依据。

Nasopharyngeal carcinoma is one of the most common head and neck malignant tumors in Guangxi . Studies have shown the regulatory role of miR - 126 -VEGF had an important role in occurrence and development of different types of tumors but its role in nasopharyngeal carcinoma has rarely been reported .Research from a new set of matrine derivatives, found the derivative YYJ18 has stronger effect in the inhibition of the growth of nasopharyngeal carcinoma cells than matrine, and found that the changes in ERK1/2, p38 and Akt signal pathway is related to this mechanism. For the further study of the role matrine and matrine derivatives, its target and differences in Nasopharyngeal carcinoma cells, through the introduction of exogenous miR - 126 in nasopharyngeal carcinoma cells, observe the regulatory role of miR - 126 in VEGF - MAPK/Akt pathways. At the same time, to discuss whether matrine and matrine derivatives by intervening in miR - 126 - VEGF regulation, affect the MAPK/Akt signal pathway, and induce apoptosis , inhibition of cell proliferation ,invasion and metastasis of nasopharyngeal carcinoma cells . This project will be conducted on mechanism of matrine derivatives, especially the study of target gene discovery and function to provide experimental basis.