单细胞功能与行为的精准化学调控和原位分析，对于揭示细胞活动的化学本质以及生理病理过程分子机制具有重要意义。本课题以单细胞活动为研究对象，针对细胞命运精准调控和单细胞分析中的关键问题，通过新蛋白质调控工具的制备和微电极表界面的合理设计与构筑，建立细胞相关生理活性分子的高选择、时空分辨分析新方法。主要研究内容包括：1）通过蛋白质修饰或改造，构建细胞命运调控的新蛋白质工具；2）针对待测生理活性分子的物理化学性质和检测需求，建立单细胞电化学分析的新原理和新方法；3）结合蛋白质参与的细胞命运调控，验证所建立新方法在细胞功能与行为分析中的适用性。本项目的研究不仅将提高细胞命运调控的精准度，还将推动相关生理病理过程中分子机制和动态变化规律的研究。

The accurate modulation of cell function and behavior and the real-time measurement of chemical dynamics are significant to revealing the chemical essence of cell activities and establishing molecular mechanisms of the physiological and pathological processes. Combining cell fate modulation with the protein toolbox and the rational engineering of electrochemical interface, this project will develop a new analytical strategy for detecting physiologically active molecules with high selectivity and spatiotemporal resolution. The main content of this project includes: 1) expanding the toolbox for cell fate modulation by protein chemical modification or protein remolding; 2) establishing new electrochemical analytic methods for single cell analysis; 3) validating the applicability of the established sensing strategy in combination of protein modulation for exploring cell function and behavior. This research will not only improve the accuracy for cell fate manipulation, but also promote the molecular investigation of physiological and pathological processes.