饮食所致脂肪过度积累是机体代谢失衡的风险因素。近年研究发现，膳食多酚可诱导白色脂肪棕色化，从而促进脂肪消耗并成为改善代谢失衡的有效策略，但其分子机制尚未完全阐明。申请人近期获得重要线索，石榴多酚可诱导白色脂肪棕色化，并研究获得线粒体外膜蛋白mitoNEET可能参与启动这一过程，其表达可能受miR-155-5p等miRNA的调节。由此本课题研究假设：石榴多酚可特异性调节白色脂肪miRNA水平，进而激活mitoNEET介导的白色脂肪棕色化。本课题拟首先解析石榴多酚在诱导白色脂肪棕色化过程中调控mitoNEET表达的时效关系；其次，沉默mitoNEET基因表达，分析其在石榴多酚诱导白色脂肪棕色化中的作用；最后，利用筛选获得的miRNA，探索石榴多酚通过miRNA调控mitoNEET表达的分子机制。该课题将有望揭示石榴多酚诱导白色脂肪棕色化的作用机理，为膳食多酚改善饮食所致代谢失衡提供新理论依据。

Diet-induced excessive fat accumulation is the risk factor for metabolic imbalance. Recent studies have found that dietary polyphenols can induce white fat browning and promote fat consumption, which has become an effective strategy to improve metabolic imbalance, but its molecular mechanism has not been fully elucidated. The applicant recently obtained important clues that pomegranate polyphenols can induce white fat browning, and studied that the mitochondrial outer membrane protein mitoNEET may be involved in initiating this process, and the expression of mitoNEET may be regulated by miRNAs such as miR-155-5p. Therefore, this study hypothesized that pomegranate polyphenols can specifically regulate the level of miRNAs in white fat, thereby activating mitoNEET-mediated white fat browning. This study will first analyze the time-effect relationship of pomegranate polyphenols in regulating mitoNEET expression during the induction of white fat browning; Second, silence mitoNEET gene expression and analyze its role in pomegranate polyphenol-induced white fat browning; Finally, the screened miRNA was used to explore the molecular mechanism of pomegranate polyphenols regulate mitoNEET expression through miRNA. This study is expected to reveal the mechanism of pomegranate polyphenols in inducing white fat browning and provide a new theoretical basis for dietary polyphenols to improve metabolic imbalances.