白头翁汤为中兽医临床治疗湿热泄泻经典名方，但对其生物效应机制尚不完全清楚。本项目拟以白头翁汤对湿热泄泻模型进行干预，运用代谢组学方法，采用超高压液相色谱-飞行时间质谱法(UPLC/Q-TOF-MS)和气质-联用(GC-MS)分析血浆、尿液、结肠及回肠的代谢物整体变化情况，研究白头翁汤干预湿热泄泻模型的代谢指纹图谱，筛选白头翁汤干预湿热泄泻的纠偏代谢物；利用京都基因与基因组百科全书(KEGG)数据库构建纠偏代谢物的生物通路关联网络。在此基础上，采用网络分析和编辑软件Cytoscape 对白头翁汤干预湿热泄泻的纠偏代谢物、通路、网络靶标进行分子对接，建立白头翁汤代谢物-通路-靶点网络，阐述白头翁汤干预湿热泄泻的生物分子网络交互作用，从“点-线-面”多维度分析白头翁汤与机体之间的相互作用关系，系统地、全面地揭示白头翁汤治疗湿热泄泻的生物效应机制，为湿热泄泻的治疗和中兽药的研究及临床应用提供依据。

Baitouweng Tang is famous prescription for veterinary clinical treatment diarrhea due to damp-heat, widely used, but its mechanism for the treatment of diarrhea due to damp-heat syndrome is not clear. Referenced the idea of the project network pharmacology, Baitouweng Tang as object, the intervention on diarrhea due to damp-heat syndrome animal model, the use of metabonomics research methods, the ultrahigh pressure liquid chromatography - time of flight mass spectrometry (UPLC/Q - TOF - MS) and temperament - combination (GC - MS) detection of urine, blood plasma, tissue metabolites, the change of the whole, establish metabolic fingerprint screening of Baitouweng Tang and intervention metabolic marker of diarrhea due to damp-heat syndrome; Using KEGG database construction of biological pathways associated metabolic markers of network. On this basis, the intervention of Baitouweng Tang with Cytospace software metabolite markers of diarrhea due to damp-heat syndrome, the channel, the network targets for molecular docking, establish Baitouweng Tang metabolite pathways - target network, this paper Baitouweng Tang intervention diarrhea due to damp-heat syndrome of molecular interaction network, from "point - line - face" to establish a multi-dimensional the interaction relationship between the body and blood tonic soup, to systematically and comprehensively explain the mechanism of action, clinical application and Baitouwang Tang in veterinary medicine provides the basis for new drug research, etc.