腹泻是目前养殖业中亟待解决的一个重要课题，白头翁汤是临床治疗湿热型腹泻的名方，但作用机制不明确。前期研究发现白头翁汤可以通过调节胆汁酸的代谢治疗湿热型腹泻，但具体胆汁酸如何调节机体以及与肠道菌群的相互作用不清楚。因此，本项目以白头翁汤为研究对象，建立湿热泄泻动物模型，以“胆汁酸靶标代谢监测”为切入点，拟采用基因表达谱分析、靶标代谢组学、宏基因组学等方法分析白头翁干预湿热泄泻大鼠肝组织中胆汁酸通路相关基因的表达，血清、肝及肠组织中胆汁酸组分的含量，肠道菌群的变化。通过对以上数据的相关及多重比较揭示胆汁酸通路相关基因表达、胆汁酸组分的含量及肠道菌群变化之间内在的规律及与湿热泄泻的关系，以阐明白头翁汤治疗湿热泄泻的 "肝－胆汁酸－肠道菌群"轴生物效应机制，为系统、全面阐释白头翁汤的作用机制、临床应用和湿热型腹泻治疗等方面提供依据。

Diarrhea is an important subject to be solved in the breeding. Baitouweng Tang is famous prescription for veterinary clinical treatment diarrhea, widely used, but its mechanism for the treatment of dampness-heat diarrhea syndrome is not clear. According to Traditional Chinese veterinary Medicine theory and clinical practice and the previous study which discover Baitouweng Tang treat the Diarrhea by adjusting the bile acid，the Baitouweng Tang as object, the intervention on dampness-heat diarrhea syndrome animal model, we analyze bile acid pathway related gene expression in liver tissue, bile acid quantitative monitoring and intestinal flora changes by using the use of Gene expression spectrum analysis, target metabolomics and macro genomics. After analyzing several of the integration of the above data study bile acid metabolism related gene expression in liver tissue, bile acid in the serum and liver tissue and intestinal flora between inner rule changes, we except to reveal the gene expression patterns changes, bile acid metabolism pathway, the relationship between intestinal flora changes and diarrhea, to clarify Baitouweng Tang in the treatment of diarrhea liver-bile acid-intestinal axis mechanism, focus on the future basic experiments and clinical experiments on the Baitouweng Tang treatment of intestinal flora as target accurately and single optimization, making it more effective pulsatilla decoction, giving full play to the advantages of Traditional Chinese Veterinary Medicine.