micro RNA参与糖尿病及其并发症的发病过程。NOX4已被证明是血管内皮细胞ROS产生的主要来源，ROS及其引发的内皮细胞损伤是糖尿病血管病变的始动因素。micro RNA参与糖尿病及其并发症的发病过程，研究显示miR-146a可能与NOX4相关。因此，我们提出假说：氧化应激损伤内皮细胞中miR-146a的表达增加，导致NOX4减少，ROS升高和细胞凋亡等变化。本研究拟采用人脐静脉内皮细胞及糖尿病大鼠模型，通过RNA干扰、Realtime PCR 、Western blot等方法，从分子、细胞及整体水平分析miR-146a在内皮细胞氧化损伤中的作用，探讨miR-146a改善内皮细胞功能紊乱，减少细胞凋亡的调控机制，为糖尿病大血管病变提供新视角。

microRNAs involve in the pathogenesis of diabetes and its complications. It has been proved that in vascular endothelial NOX4 is the main resource of ROS which can damage endothelial and result in diabetes vasculopathy.Recent study have shown the level of miR-146 decreased and the express level of its target gene-NOX4 rise in endothelial. Then,we have the hypothesis: decrease of the level of miR-146 in endothelial damaged by oxidative stress regulates the rise of NOX4 accompany with changes such as cell apoptosis and so on. In the present study,we will examin the protective effects of miR-146a on endothlial injury and the mechanism of miR-146a to improve endothelial dysfunction andreduce cell apoptosis in human umbilical vein endothelial cells (HUVEC)s and rat model of diabetes, using RNA interference, Realtime PCR, Western blot and other methods, from molecules, cells, tissues and animals, and other aspects of the overall level to analyze the role of miR-146a on endothelial cell injury of glucolipotoxicity. This study will explore the role of microRNA in the vascular complications of type 2 diabetes prevention and treatment from a new perspective.