颞下颌关节(TMJ)假体是修复重建晚期TMJ病损的有效方法，且利用聚醚醚酮(PEEK)制作TMJ假体已逐步成为口腔医学研究的热点。如何增强PEEK力学性能和成骨活性是其能否用于TMJ假体的关键要素。基于前期制备的碳纳米管(CNTs)增强型PEEK复合材料和羟基磷灰石(HA)沉积于CNTs-PEEK表面技术，本项目拟根据TMJ假体力学要求深度优化CNTs-PEEK的超声处理复合技术和3D打印工艺；进一步构建CNTs-PEEK多孔结构沉积HA的骨诱导性界面，使其能与颌骨形成强有力的骨结合；再与牙槽骨干细胞(Al-BMSCs)共培养后检测其对细胞骨向分化的影响，并采用高通量定量蛋白质组学揭示其促进细胞骨向分化的机制；最后制备个性化CNTs-PEEK-TMJ假体并完成骨诱导性表面处理，在体外力学检测和大动物体内验证其性能。希望本项目能为CNTs-PEEK在TMJ假体的应用奠定基础。

Temporomandibular joint (TMJ) prosthesis is an effective method to treat end-staged TMJ lesions, and the application of polyetheretherketone (PEEK) in TMJ prosthesis has becoming a research focus in stomatology. How to enhance the mechanical strength and improve the osteogenic activity of PEEK are the two important problems to achieve this goal. Based on our previously synthetic carbon nanotubes (CNTs) reinforced PEEK composite and hydroxyapatite (HA) deposited on CNTs-PEEK surface, this project will firstly plan to deeply optimize ultrasonic treatment and 3D printing process for CNTs-PEEK composite preparation according to the mechanical requirements of TMJ prosthesis, and further build an osteoinductive interface which could form a strong bone integration with the mandible by porous CNTs-PEEK structure and HA deposition technique. Then, the osteoinductive interface is cultured with the alveolar bone marrow stromal cells (Al-BMSCs) to uncover its ability and the mechanism of promoting osteogenic differentiation of Al-BMSCs by proteomics. Finally, the customized CNTs-PEEK-TMJ prosthesis with osteoinductive surface in the bone contact side is manufactured and verified in vitro and vivo. It is hoped that this project can lay a foundation for the application of CNTs-PEEK in TMJ prosthesis.