探究调节性T细胞在异体移植复合组织瓣内的外周神经修复作用
结题报告
批准号:
82002064
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
许恒
依托单位:
学科分类:
体表组织器官移植与再造
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
许恒
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中文摘要
外周神经功能预后效果不佳是阻碍异体复合组织瓣移植(VCA)开展的主要因素之一。寻找抗免疫排异有益,并促进外周神经修复的策略是本领域的研究焦点。近年,大量研究证明调节性T细胞(Treg)不仅可抗免疫排异(如VCA),而且可修复损伤组织。我们前期研究发现扩增Treg不仅延长VCA的存活时间,而且改善外周神经功能的恢复。因此推测外周神经损伤后,Treg响应、迁移并聚集在损伤的外周神经组织内参与修复。本项目拟应用Rag1-/-小鼠过继Treg探究其外周神经修复作用的特异性;然后应用自主开发的近红外II区成像技术活体定位Treg细胞的迁移和分布,识别其与VCA外周神经肿胀/消退等形态变化的关系;最后调节Treg的功能,结合外周神经组织学、行为学明确Treg在VCA中的外周神经修复作用。本研究将揭示Treg在VCA外周神经损伤修复中重要作用,为改善VCA外周神经功能预后,提高患者生活质量提供新思路。
英文摘要
Poor prognosis of peripheral nerve function is one of the main factors hindering the development of vascularized composite allotransplantation (VCA). The research focus of this field is to find the strategy of both achieving anti-immune rejection and repairing peripheral nerve. In recent years, numerous studies have demonstrated that regulatory T cells (Tregs) can not only be benefit for anti-immune rejection (e.g., VCA), but also repairing injured tissues. Our previous study found that amplifying the Tregs not only elongated the survival time of VCA, but also improved the recovery of peripheral nerve function. Therefore, it is speculated that after peripheral nerve injury, Tregs respond, migrate and gather around the injured peripheral nerve tissue to participate in repair. This project intends to explore the specificity of the Treg repair function involved in peripheral nerves by using adoptive Tregs strategy in Rag1 - / - mice; then apply the independently developed near-infrared II imaging to pinpoint the migration and distribution of Tregs involved in peripheral nerve in living animal, the aim of which is to identify the relationship between nerve morphological changes and Tregs behavior; finally, by modulating the function of Treg, combined with peripheral nerve histology and behavioral tests, we further clarify the function of regulatory T cells in repairing peripheral nerve in VCA. This study will reveal the important role of regulatory T cells in repairing peripheral nerve in VCA, providing new ideas for improving the prognosis of peripheral nerve function of VCA and the quality of life of patients.
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DOI:10.1111/exd.14700
发表时间:2022-10
期刊:Experimental Dermatology
影响因子:3.6
作者:Yeltai Nurzat;Zhu Zhu-Zhu;Yixin Zhang;Heng Xu
通讯作者:Yeltai Nurzat;Zhu Zhu-Zhu;Yixin Zhang;Heng Xu
DOI:10.1002/term.3264
发表时间:2021-11
期刊:Journal of Tissue Engineering and Regenerative Medicine
影响因子:3.3
作者:Zhu Zhu-Zhu;Linxiumei Guo;Yeltai Nurzat;Heng Xu;Yixin Zhang
通讯作者:Zhu Zhu-Zhu;Linxiumei Guo;Yeltai Nurzat;Heng Xu;Yixin Zhang
DOI:10.2147/ijgm.s337951
发表时间:2022
期刊:International journal of general medicine
影响因子:2.3
作者:Nurzati Y;Zhu Z;Xu H;Zhang Y
通讯作者:Zhang Y
DOI:10.1111/wrr.13086
发表时间:2023-05
期刊:Wound Repair and Regeneration
影响因子:2.9
作者:Linxiumei Guo;Zhu Zhu-Zhu;Chenchen Shi;Yuxin Jia;Yeltai Nurzat;W. Su;Yixin Zhang;Heng Xu
通讯作者:Linxiumei Guo;Zhu Zhu-Zhu;Chenchen Shi;Yuxin Jia;Yeltai Nurzat;W. Su;Yixin Zhang;Heng Xu
DOI:10.1038/s41423-021-00834-1
发表时间:2022-01
期刊:Cellular & Molecular Immunology
影响因子:24.1
作者:Heng Xu;Zhu Zhu-Zhu;Jian Hu;Jiawei Sun;Yan Wo;Xianshu Wang;Hongzhi Zou;Bin Li;Yixin Zhang
通讯作者:Heng Xu;Zhu Zhu-Zhu;Jian Hu;Jiawei Sun;Yan Wo;Xianshu Wang;Hongzhi Zou;Bin Li;Yixin Zhang
国内基金
海外基金