激痛点是导致肌肉疼痛和功能障碍的主要根源，由于急慢性损伤造成的细胞膜受损使胞内钙离子浓度增高，导致肌节挛缩出现收缩结，属于中医筋结范畴。对激痛点去活化就是舒张挛缩的肌节以消除收缩结，中医用舒筋解结来阐述。舒筋是推拿治病的重要机制之一，是该领域内研究的热点和难点。目前，对舒筋的机制研究集中在胞内钙离子的调节上，而DHPR/RyR1对钙离子的调节是肌节收缩和舒张功能的关键。本项目以此为切入点，以慢性激痛点模型大鼠为受试对象，用按法刺激，运用行为学、形态学、肌电、肌骨超声、免疫组化、WB等检测手段，通过观察激痛点局部痛阈、组织硬度、自发电位，组织形态变化，以及肌细胞膜机械敏感通道相关蛋白、胞内钙调控蛋白、钙离子信号通路相关蛋白、DHPR/RYR表达的变化，从DHPR/RYR1对胞内钙离子的调节角度，揭示按法的机械刺激作用于肌细胞通过胞内信号再调节DHPR/RYR1的表达来发挥舒筋解结的效应机制。

MTrPs is the main cause of muscle pain and dysfunction, which is caused by increased intracellular calcium ion concentration due to acute and chronic injury, resulting in Contraction Knot of sarcomere contracture, which belongs to the category of Chinese medicine Muscles Nodule.The deactivation of MTrPs is to release the Contraction Knot by relax sarcomere, it is illustrated by Relax Muscles ＆Relieve Nodule in Chinese medicine. Muscle Relaxation is one of the important mechanisms of Tuina therapy, which is the hot and difficult point in this field.At present, research on Muscle Relaxation has focus on the regulation of intracellular calcium ions, while DHPR/RyR1 on the regulation of calcium ions is the key to sarcomere contraction and relaxation function.Based on it, model rats as the subjects, Pressing Manipulation stimulation, behavior, morphology, myoelectricity, musculoskeletal ultrasound, IHC, WB and other testing is used in this project. Observe local pain threshold, tissue hardenability, SEA, tissue morphological change. And the change of muscle cell membrane mechanical sensitive channel related proteins, intracellular calcium regulation proteins, calcium signaling pathways related proteins, DHPR/RYR expression. To reveal the effect mechanism of Relax Muscles ＆Relieve Nodule of Pressing Manipulation mechanical stimulation on muscle cells to regulation DHPR/RYR1 expression by intracellular signal,from view of regulation of intracellular calcium ion by DHPR/RYR1.