睾丸间质细胞衰老引起的睾酮（T）水平下降是男性更年期综合征主要发病基础；肝细胞生长因子（HGF）是肝源性细胞因子，能促进睾丸间质细胞T合成。本项目根据男性更年期综合征中医"肾虚肝郁"病机特点，以"乙癸同源"理论为切入点，采用"肾肝同治"法进行干预，围绕HGF分泌、睾丸间质细胞凋亡、T合成相关酶活性等问题，应用动物模型、肝非实质细胞-睾丸间质细胞共培养、细胞转染等方法，开展以下研究：①"HGF是乙癸同源物质基础"假说验证；②肾肝同治法促间质细胞T合成、干预男性更年期综合征的分子机制；③肾肝同治法干预男性更年期综合征的作用靶点。本项目通过深入研究肾肝同治法促HGF分泌、抑制间质细胞凋亡、活化T合成相关酶等机制，为临床运用肾肝同治法治疗男性更年期综合征提供实验依据，同时"HGF是乙癸同源物质基础"假说的提出与验证，将进一步丰富乙癸同源理论的科学内涵。

The basic pathogenesis of male climacteric syndrome is reduce of T level because of testis leydig cell aging; hepatocyte growth factor(HGF) is a kind of cell factor which source of liver,can Promote leydig cell testosterone synthesis.The project according to "Shen Xu Gan Yu" of male climacteric syndrome, as the breakthrough point to "Yi-Guei-Tong-Yuan",intervene in "Shen-Gan-Tong-Zhi",around this Problem about HGF secrete,leydig cell apoptosis,the activity of testosterone synthetize enzyme,using animal model,hepatic nonparenchymal cells-leydig cell cocultivation, cell transfection methods,develop some reserch: ①Proving the hypothesis of HGF is material base of Yi-Guei-Tong-Yuan; ②The effect evaluate of Shen-Gan-Tong-Zhi to leydig cell Testosterone generation and intervene male climacteric syndrome；The target of shen-gan-tong-zhi intervene male climacteric syndrome.By the research HGF secretion,leydig cell apoptosis,the activity of testosterone synthetize enzyme will show the The Experimental Base of "Shen-Gan-Tong-Zhi" to male climacteric syndrome,and A abundant the Scientific Connotation about "Yi-Guei-Tong-Yuan".