基于外泌体通讯探讨LOH发病的睾酮合成微环境机制及雄蚕益肾方干预研究

迟发性性腺功能减退症（LOH）睾酮水平低下与睾丸微环境老化有关，微环境中Sertoli细胞可能通过外泌体通讯调控Leydig细胞睾酮合成。项目组前期体内、外实验证实雄蚕益肾方能改善Leydig细胞功能，但不清楚是否影响微环境中Sertoli-Leydig细胞通讯。基于此，我们提出雄蚕益肾方可能调节Sertoli细胞外泌体分泌，影响Sertoli-Leydig细胞通讯，改善睾酮合成微环境，调控Leydig细胞功能。为了验证该假说，拟开展：①Sertoli-Leydig细胞共培养对Leydig细胞功能影响；②雄蚕益肾方通过Sertoli细胞外泌体调控Leydig细胞睾酮合成；③雄蚕益肾方通过Sertoli细胞外泌体miRNA调控Leydig细胞自噬；④雄蚕益肾方干预LOH大鼠睾酮合成的外泌体机制。以上假说验证及机制研究将完善雄蚕益肾方干预LOH的实验依据，并丰富和发展中医“整体观”科学内涵。

The low level of testosterone in Late-onset hypogonadism in males (LOH) is related to the aging of testicular microenvironment, in which Sertoli cells may regulate the synthesis of testosterone in Leydig cells through the communication of exosomes. In the early stage of the project, it was verified in vivo and in vitro that Xiongcan Yishen Prescription (XYP) could improve the function of Leydig cells, but it was not clear whether it would affect the communication of Sertoli-Leydig cells in the microenvironment. Based on the fact, we propose that the XYP can affect the communication of Sertoli-Leydig cells, improve the microenvironment of testosterone synthesis and regulate the function of Leydig cells by regulating the secretion of Sertoli cells exosomes(SCs-exosomes). To test our hypothesis, we plan to study：①The effect of Sertoli-Leydig cells co-culture on Leydig cell function; ②XYP regulates Leydig cells testosterone synthesis through SCs-exosomes; ③XYP regulates Leydig cells autophagy through SCs-exosomes miRNA; ④XYP interferes with the exosomes mechanism of testosterone synthesis in LOH rats. The above hypothesis verification and mechanism research will improve the experimental basis of XYP intervention in LOH, and enrich the scientific connotation of "holistic view" of Traditional Chinese Medicine.