维吾尔族慢性淋巴细胞白血病microRNA表达谱筛选验证及其相关分子生物学发病机制的研究

慢性淋巴细胞白血病(CLL)是一种成熟B淋巴细胞增殖性肿瘤，其发病机制尚未明确，研究发现微小RNA的异常表达在CLL的发病及预后中起重要作用。国内外研究显示不同种族CLL患者在基因的选择上有不同的偏向性。维吾尔族与汉族具有不同的遗传背景，推测分子生物学在其疾病发生及预后可能存在不同的影响。课题组应用miRNA 芯片对维吾尔族CLL患者及正常人测序，筛选维吾尔族CLL患者差异表达的miRNA，在相关数据库查找与CLL密切相关的差异表达miRNA靶基因，通过体外萤光素报告载体验证预测靶基因，并检测miRNA对靶基因、甲基化敏感基因的表达、调控序列甲基化状态的影响。通过上述研究揭示miRNA异常表达在维吾尔族CLL患者的发病机制中的作用，并比较上述指标与国内其他人群的差异性。分析miRNA与目前已知预后指标的相关性并探讨其预后意义，为新疆维吾尔族CLL患者今后的靶向治疗提供科学实验基础。

Chronic lymphocytic leukemia (CLL) is a kind of mature B lymphocytes proliferation of tumor, the pathogenesis is still not clear, the study show miRNA expressing may play an important role in the pathogenesis and prognosis of CLL.Domestic studies show that patients of different races have different CLL bias in gene selection.Uighurs and Han have a different genetic background , molecular biology in their disease occurrence and prognosis may exist different performance and impact.Research group sequence Uygur CLL patients and normal individuals by miRNA microarray, Selected Xinjiang Uygur CLL patients differentially expressed miRNA, find the differential expression of miRNA target genes in the international authoritative database, verificate the predicted target gene by vitro luciferase reporter vector, and detecte the miRNA target genes and methylation sensitive gene expression and. regulatory sequence methylation status. Through the above research reveals the abnormal expression of miRNA in the pathogenesis of Uygur in CLL, and compare these indicators with other populations differences. Analysis the miRNA correlation with known prognostic indicators and to investigate the prognostic significance, provide a theoretical basis for the new target for the treatment of CLL.