甘蓝型油菜半矮杆材料DW871特异型株高及花序性状连锁基因的QTL精细定位

油菜倒伏主要原因是株高，具有株高半矮秆、特别适宜机械化生产的油菜种质资源鲜见报道。前期，项目组创制的甘蓝型油菜半矮杆材料DW871，具株高中等（140cm），花序散生，分枝紧凑（＜25°)、着果密（1.86个/厘米）、茎杆木质化程度高、分枝挺直不串枝、高抗倒伏、收获指数高（0.37）、抗裂角性强，且株高和花序性状紧密连锁，特适宜于机械化生产等优点。初步认为DW871株高及花序性状主要受1对显性主基因控制，位于A10连锁群。为精细定位与DW871的株高及花序性状连锁的基因，本研究拟构建与株高及花序性状连锁的分离群体，以Seq-BSA分析方法，进一步发掘与DW871株高及花序性状关联候选SNP标记，构建高密度遗传图谱，对株高及花序性状基因进行精细定位并确定候选基因，通过比较，明确与DW871株高及花序性状连锁的基因是否为新基因，研究结果对加速半矮杆材料DW871的相关优势性状基因应用意义重大。

Plant-height is the main factor for the lodging of Brassica napus. DW871 is a new semi-dwarf germplasm resource of Brassica napus, which have many good features, such as a medium height, compact plant phenotype, completely lodging resistance, no cross branchs, silique with good crack-resistance and especially suitable for mechanized cultivation. The preliminary genetic analysis showed that the plant-height trait of DW871 was controlled by a pair of dominant genes. So far, there are few reports about the semi-dwarf Brassica napus with medium plant-height which is suitable for mechanized cultivation. In this study, the gene which control the DW871 plant-height will be quickly and accurately located through Cluster Separation Analysis (Seq-BSA) which based on the second generation sequencing technology. Firstly, The F1 and F2 populations will be obtained by hybridization with semi-dwarf DW871 and normal varieties with high plant height, then the plant-height pool will be constructed according to plant-height traits, and initially determine the location of the marker on chromosome. Secondly, further to verify the molecular markers which are closely linked to plant-height traits by scanning all F2 populations with the screened out markers, and construct high-density genetic map. Thirdly, determine whether the plant-height gene of DW871 is a new gene through the comparison with the known dwarf-plant-height genes which are located on the same chromosome. The research results about the plant-height trait of DW871 will accelerate the breeding and utilization of the mechanized cultivars of Brassica napus.