油菜含油量除受自身基因型遗传之外，环境因素也有较大影响。油菜含油量环境钝感基因型材料筛选，是促进高油优质品种推广应用的重要基础。目前甘蓝型油菜环境钝感基因型相关基因的QTL研究及克隆在国内外均未见报道。本项目以前期定位的含油量环境钝感基因型相关基因的QTL和含油量环境差异表达基因为基础，选择含油量环境钝感基因型和环境敏感基因型且在特定染色体上发生交换的单株构建精细定位DH群体，通过增加SSR、SRAP等标记加密连锁图谱，结合在不同环境的高油DH群体含油量稳定性鉴定结果，对特定区段进行含油量环境钝感基因型相关基因的QTL精细定位，参考含油量环境差异表达谱分析结果和同源性分析及序列比较，初步筛选并克隆出含油量环境钝感基因型的相关候选基因，为开展甘蓝型油菜高油稳油遗传、品种选育及推广应用研究提供重要理论基础。

Oil seed content was mainly controlled by genetic factor, as well as environment factors. Breeding for environment-insensitive genotypes of seed oil content is an important foundation to promote the promotion and application of high oil quality varieties. QTL mapping and cloning of environment-insensitive genotype have not been reported in Brassica napus. The project use QTL of environment-insensitive for oil seed content and genotype-related genes to choose the oil seed content of the environment-insensitive and environment-sensitive genotype and specific exchanged chromosome to construct DH population for fine mapping by SSR and SRAP markers linkage map. Combined with the result of stability of high oil DH population in different environments and refer to the result of expression profile analysis for oil seed content of different environment and homology analysis, screened and cloned oil seed content of environment-insensitive candidate genes initially. That's will provide mportant theoretical basis for the stability of Brassica napus high oil genetic varieties selection and promotion of applied research.