慢性阻塞性肺病(COPD)具有高患病率、高致残率和高死亡率的特点，严重威胁人类的生命及健康。气道黏液高分泌(MH)是影响其病情变化及预后的独立危险因素。COPD因痰伏于内、寒诱于外，中医辨证为寒饮停肺证。细辛-干姜为温肺化饮的经典药对之一，课题组前期通过网络药理学预测其治疗COPD作用机制与能量代谢、氧化应激、炎症反应有关；可能通过PI3K-Akt 、VEGF、AMPK等信号通路发挥药效。进一步开展细辛-干姜水煎液治疗COPD大鼠模型动物实验，经高通量转录组测序分析，发现其确实能下调PI3K mRNA表达，并与VEGF、AMPK等信号通路密切相关，以此拮抗COPD-MH的重要生物学标志物MUC5AC而发挥药效。本项目拟在此基础上，以多组学整合研究为策略，将转录组学-代谢组学数据、指纹图谱-代谢物谱数据关联分析验证细辛-干姜药效物质基础与作用机制，为指导临床精准合理使用该药对提供科学依据。

Chronic obstructive pulmonary disease (COPD) has the features of high morbidity, high disability and high mortality, which seriously endangers the lives and health of human beings. Airway mucus hypersecretion (MH) is a independent hazard affecting the progress and prognosis of COPD. COPD is induced by sputum in vivo and cold in vitro, and the pattern of TCM syndrome differentiation is cold fluid-retention suspend in lung. Asarum-dried ginger is one of the classic drug pairs of lung-warming decoction. Our previous studies predicted that the mechanism of action in the treatment of COPD was related to energy metabolism, oxidative stress and inflammatory reaction through network pharmacology. It may play a role through PI3K-Akt, VEGF, AMPK and other signaling pathways. Further studies on the treatment of COPD by asarum-dried ginger water decoction in rat models were conducted. Through high-throughput transcriptome sequencing analysis, it was found that the decoction indeed down-regulate the expression of PI3K mRNA, and is closely related to VEGF, AMPK and other signaling pathways, so as to antagonize the important biological marker MUC5AC of COPD-MH and exert its efficacy. Base on it, this project intends to use multi-histological integration study as a strategy to conduct correlation analysis of transcriptome-metabolomics data, fingerprint-metabolite spectrum data to verify the pharmacodynamic material basis and mechanism of asarum-dried ginger, thus to provide scientific basis for guiding clinical accurate and reasonable use of the drug pair.