侧流层析传感器是一种重要的快速检测技术，具有耗时短、成本低、易操作、应用广泛等显著优势，在新冠肺炎疫情防控期间也发挥了重要作用。但现有侧流层析传感器无法实现对金黄色葡萄球菌的标准化快速检测，造成这种困境的根本原因在于国家标准要求进行“分级”检出而非不得检出，且分级界限低至100CFU/g，导致现有方法存在分级界限的盲区和灵敏度不足等瓶颈。本项目拟以牛奶中金黄色葡萄球菌为研究对象，首先构建基于“决策树”的快速分级定量模型，再分别从“单原子纳米酶的两级信号增敏”和“抵抗牛奶基质污损的识别降噪”两方面协同探究侧流层析传感器“增敏降噪”机制的关键科学问题，最终探索出一种标准化判定牛奶中金黄色葡萄球菌是否合格的快速分级检测方法。本项目旨在寻求侧流层析传感技术在牛奶中金黄色葡萄球菌快速检测领域的突破，从而提升该技术对标准培养法检测的辅助能力，为满足我国监管部门日常检测和筛查工作的实际需求提供科学依据。

Lateral flow biosensor is an important technique for rapid detection, which has obvious advantages such as short time consumption, low-cost and easy operation. It also has played a key role in the prevention and control of the coronavirus disease 2019 (COVID-19) pandemic. However, all existing lateral flow biosensing methods could not perform the standardized rapid detection of Staphylococcus aureus. Because it needs “graded” detection instead of not allowed detection according to national standard. Additionally, the regulated limit of national standard is as low as 100CFU/g. Therefore, the graded blind zone and low-sensitivity bottleneck caused the predicament. This project will take Staphylococcus aureus in milk as the research object. Firstly, it is proposed to establish a fast graded quantification model based on “decision tree”. Then, it is planned to explore the scientific issues of “increasing sensitivity and reducing noise” on lateral flow biosensor from two aspects of “the two-level signal increasing sensitivity mechanism of single-atom nanozyme” and “the reducing noise identification mechanism to resist matrix fouling in milk”, respectively. Finally, a rapid and graded detection method that can be standardized to determine whether it is qualified of Staphylococcus aureus in milk will be explored. The goal of this study is to seek a breakthrough in the lateral flow biosensor for rapid detection of Staphylococcus aureus in milk. It is hoped that the proposed assay will improve the auxiliary ability of the standard culture method, and then provide a scientific basis for meeting the actual needs of the daily inspection and screening work of China's regulatory authorities.