鸡毒支原体（MG）引起的慢性呼吸道病（CRD）是鸡的重要传染病，给养禽业造成巨大的经济损失。研究表明，外泌体miRNAs能启动机体免疫及炎症反应调控病原感染。课题组前期研究发现，MG-HS感染的鸡Ⅱ型肺泡细胞（ATⅡ）外泌体gga-miR-193极显著升高。本项目以gga-miR-193为切入点，利用CLIP-seq筛选外泌体gga-miR-193的直接靶基因及调控通路；通过超表达及 CRISPR/Cas9 敲低/除技术，采用ATⅡ、鸡胚成纤维细胞系（DF-1）和鸡巨噬细胞系(HD-11)等研究外泌体gga-miR-193/靶基因调控机体MG-HS感染的分子机制；构建鸡胚模型确证gga-miR-193/靶基因的调控功能。项目的完成将诠释外泌体gga-miR-193在MG-HS感染中的作用及其分子机制，为研发抗MG-HS感染的新型药物及综合防控技术提供科学依据。

Chronic respiratory disease (CRD) caused by Mycoplasma gallisepticum (MG) is one of the most important infectious diseases in chickens, which causes huge economic losses to poultry industry. Studies have shown that exosome microRNAs can activate host immune and inflammatory responses to regulate pathogen infection. Previous studies from our laboratory showed that gga-miR-193 was significantly up-regulated in the exosome of chicken alveolar cell typeⅡ (ATⅡ) infected with MG-HS. Therefore, this project uses gga-miR-193 as the entry point, using CLIP-seq method to screen the direct target gene of exosome-gga-miR-193 and their association regulatory network; employing overexpression and CRISPR/Cas9 knockdown or knockout techniques to study the molecular mechanism of exosome gga-miR-193/target gene regulate MG-HS infection using ATⅡ, Chicken embryo fibroblast (DF-1), and chicken macrophage cell lines (HD-11); moreover, a chicken embryo model will be constructed to further confirm the regulatory function of gga-miR-193/target gene in vivo. The completion of the project will elucidate the function and molecular mechanism of exosome gga-miR-193 regulating MG-HS infection, and provide a scientific basis for the development of new drugs and comprehensive prevention and control technologies against MG-HS infection.