木霉生物有机肥能显著促进植物生长并有效防控土传病害，合理施用是减少我国农用化学品投入的重要途径之一。但生产过程存在一系列的技术瓶颈，其中pH的控制和秸秆分解能力尤为关键，低pH（3.0）可以降低杂菌的污染，而秸秆快速分解有利于其菌丝生长和产孢。本研究拟采用高能射线随机突变的方法，筛选出低pH条件下高效分解秸秆的突变株哈茨木霉M3；通过同源重组单基因突变和多基因突变的方法获得不同功能突变株，并结合qPCR、酶学、蛋白质电泳和组学方法比较分析各突变株和原始菌株在低pH条件下秸秆利用的差异，验证哈茨木霉M3“通过稳定细胞膜结构和能量补偿方式实现低pH下木质纤维高效降解”的假说。研究结果将为揭示哈茨木霉木质纤维降解酶分泌调控的分子机制、突破木霉类生物有机肥制造瓶颈提供理论依据，更为我国农业生产过程中作物秸秆的资源化利用和农业可持续发展提供技术支撑。

Trichoderma based bio-organic fertilizer can significantly promote plant growth and effectively prevent and control soil-borne diseases, and the rational application of these products is one of the important ways to reduce the input of agricultural chemicals in China. However, there are a series of technical bottlenecks during the production process, among which pH control and straw decomposition ability are particularly critical, and low pH (3.0)can reduce the contamination and the rapid decomposition of straw is conducive to its growth and sporulation. This study intends to screen for mutants that efficiently decompose straw under low pH conditions through a high-energy ray random mutation method. Simultaneously, different functional mutants were obtained by homologous recombination of single gene mutations and multi-gene mutations, and qPCR, enzymology, protein electrophoresis and omics methods were used to analyze the differences during lignocellulose utilization between mutants and original strains under low pH conditions, which will verify the hypothesis that T. harzianum can achieves efficient degradation of lignocellulose at low pH through cell membrane structure stabilization and energy compensation. The results will provide a theoretical basis for revealing the molecular regulation mechanism of lignocellulolytic enzymes secretion and breaking through the bottleneck of the Trichoderma based bio-organic fertilizers production, and provide technology for the resource utilization of crop straws and sustainable development of agriculture in China.