课题组前期研究表明，电针干预和骨髓间充质干细胞(Bone marrow mesenchymal stem cells，BMSCs)移植后皆可增加大鼠薄型子宫内膜的厚度，促进子宫内膜血管、腺体再生，二者结合效果更佳。国内外研究表明，BMSCs在体内SDF-1/CXCR4信号轴及其下游PI3K/Akt信号通路的作用下，可向损伤部位归巢、迁移，进而分化成各种细胞，促进组织再生。本项目提出“电针良性调控SDF-1/CXCR4，促进BMSCs归巢，修复损伤内膜，可能是电针结合BMSCs增强损伤子宫内膜修复的机制之一”的科学假说。实验采用无水乙醇宫腔注射法，建立薄型子宫内膜大鼠模型，对比观察电针和BMSCs对大鼠子宫内膜形态、容受性和胚胎着床的影响，并运用分子生物学检测方法及活体荧光成像技术，深入探讨电针促进BMSCs向损伤部位归巢的机制，为针灸治疗薄型子宫内膜在临床上的运用提供可靠的科学证据。

Our previous study demonstrated that electroacupuncture and BMSCs transplantation can improve the thickness of rat thin endometrium and promote endometrial vascular and gland regeneration,and the combined effect is better.Domestic and foreign research shows that BMSCs can be homing and migrating to injured sites under the action of SDF-1/CXCR4 signaling axis and downstream PI3K/Akt signaling pathway in vivo,and then differentiate into various cells for tissue regeneration.A scientific hypothesis is proposed by our project that"electroacupuncture of benign regulation of SDF-1/CXCR4,promote the homing of BMSCs,repairing the injured endometrium,may be one of the mechanism of electroacupuncture combined with BMSCs to enhance the injury of endometrial repair".This study intends to establish a thin endometrial rat model by intrauterine injection of absolute ethanol to observe the effects of electroacupuncture and BMSCs on the tissue morphology,receptivity,and embryo implantation of rat endometrium.Using molecular biological detection methods and in vivo fluorescence imaging technology,the mechanism of electro-acupuncture in promoting the homing of BMSCs to the injured site was deeply discussed.It provides reliable scientific evidence for the clinical application of acupuncture treatment for thin endometrium.