木质纤维素是地球上最丰富的可再生生物质能源，但其中的木质素难以降解成为制约其高效利用的瓶颈。子囊菌作为自然界最主要的木质素降解菌之一，其生物降解木质素的分子机制尚不明确。本项目以具有较强木质素降解性能的子囊菌Paraconiothyrium variabile GHJ-4作为研究材料，以蛋白质组学和代谢组学为主体的系统生物学方法为研究手段，查明天然杨木木质素降解过程中GHJ-4胞外大分子酶蛋白和小分子代谢物的变化；筛选出木质素降解相关酶蛋白和代谢标志物；探明GHJ-4胞外各活性组分对天然杨木木质素的降解规律；基于以上研究，从蛋白质组学和代谢组学水平上探索子囊菌Paraconiothyrium variabile GHJ-4生物降解木质素的分子机制。为全面揭示自然界子囊菌降解木质素的作用机制提供理论依据，为新型木质素降解反应体系的重新构建和生物质资源的高效利用提供有益的参考。

Lignocellulose is the most abundant renewable biomass resource on earth. However, the degradation of lignin is the key obstacle for transitioning lignocellulosic feedstock. Ascomycetes are one of the most efficient wood-degrading fungi，but molecular mechanism behind lignin degradation have not yet clear. In this study, we focus on investigating the molecular mechanisms of lignin degradation by ascomycete Paraconiothyrium variabile GHJ-4, having a certain lignin degradation capacity, using systems biology-based approaches involving proteomics and metabolomics techniques. On the basis of ascertaining the change of proteins and metabolites secreted by GHJ-4 during degrading natural lignin, screening relevant differential proteins and metabolites biomarker involved in lignin degradation, finding out the pattern of native lignin degradation of different components secreted by GHJ-4, and molecular mechanism behind lignin degradation in GHJ-4 through integrated analysis of proteomics and metabolomics data will be elucidated. Our findings will lay a theoretical foundation for future exploration for mechanism behind lignin degradation by ascomycetes in the nature, and also provide a useful reference for reconstructing of new lignin-degrading systems and using the available biomass resources more efficiently.