吲哚生物碱类化合物在自然界中分布广泛、其骨架类型复杂多变，具有多种多样的药理生理功能，是寻找和发现临床一线药物先导化合物的重要源泉。夹竹桃科狗牙花属(Tabernaemontana)植物作为民族药在云南傣族有广泛使用，具有重要的民间药用价值，前期研究工作表明该属植物中富含单萜吲哚生物碱类成分。申请人前期从该属植物中国狗牙花中共分离鉴定了46个单萜吲哚生物碱，包括1个具有猪笼草状的新颖骨架和7个新颖结构的天然产物，并发现部分化合物具有显著的抗微生物活性。基于此，本项目拟对我国资源丰富的三种狗牙花属植物中单萜吲哚生物碱类成分展开深入的化学成分和药理作用研究，对分离得到的化合物重点进行抗微生物活性筛选，以期获得结构新颖的活性单体化合物，并进行构效关系和作用机制探讨。该项目的申请将对狗牙花属植物资源的研究开发和先导化合物的发现具有重要的科学意义。

Indole alkaloids with the characteristics of wide distribution in nature and diverse structures, are important sources for finding and discovering drug lead compounds. Tabernaemontana species are widely used as a national medicine in the dai nationality of yunnan province and have been used for the treatment of a variety of ailments. Previous phytochemical investigations on Tabernaemontana plants had resulted in the isolation of diverse monoterpenoid indole alkaloids (MIAs) with potent antimicrobial activity, especially, 46 MIAs were isolated and identified by the applicant from Tabernaemontana chinensis, including 2 nepenthe-like indole alkaloids and 7 novel compounds, and some alkaloids were found to have significant antimicrobial activity by the applicant. Based on this, the project intends to carry out an in-depth study on the monoterpenoid indole alkaloids from three Tabernaemontana genus plants, and focus on the screening of antimicrobial activity as well as structure-activity relationships. The study of this project will be of great significance to the Tabernaemontana genus development and the discovery of drug lead compounds.