严重创伤造成粉碎性骨折，导致大段骨缺损，引起机体活动功能障碍和伴神经感觉障碍，严重影响患者生活质量。骨细胞再生微环境对于骨创伤修复至关重要，目前尚未见维护骨细胞再生微环境的人工骨架材料。项目组应用前期创新合成生物可降解的聚谷氨酸-羟基磷灰石复合管腔材料(PGA+HA)，包载具有组织修复再生能力的低等动物骨脱细胞基质(dBECM)凝胶和骨髓间充质干细胞(MSCs)，构建新型骨架材料MSCs+dBECM-PGA+HA，原位移植到骨断端处，形成紧密贴合骨组织的三维支撑型骨架，形成促进骨细胞再生的微环境，促进骨细胞的增殖、黏附和迁移。本项目通过细胞学、影像学、病理学、分子生物学等综合评价方法，研究原位移植三维骨架MSCs+dBECM-PGA+HA对于骨创伤模型动物的高效修复作用及其机制，建立综合评价体系，发挥脱细胞基质材料、干细胞移植、制剂新技术多学科技术优势，以期实现安全高效的骨创伤的修复治疗。

Comminuted bone defect caused by serious trauma can lead to serious bone defect and induce the disability of activity and sensory disturbance which will seriously affect the patients’ living quality. Bone cell regeneration microenvironment is crucial for bone repair. However, few artificial bone materials qualify the requirement for supporting bone cell regeneration microenvironment. Based on our novel synthetic material “Polyglutamic acid–hydroxyapatite (PGA+HA)”, a novel three dimensional supporting scaffold “MSCs+dBECM-PGA+HA” is constructed, which contains decellularized bone extracellular matrix (dBECM) and mesenchymal stem cells (MSCs) in its internal space. After in situ transplantation, MSCs+dBECM-PGA+HA will tightly attach to the residual bone fracture and form a three-dimensional supporting scaffold protecting the microenvironment for bone cell regeneration, which promotes the proliferation, adhesion and migration of bone cells. With the comprehensive evaluation including cytology, imaging, pathology and molecular biology, the repair effects and intrinsic mechanism of the three dimensional supporting scaffold MSCs+dBECM-PGA+HA for bone damage model animals will be investigated, and the comprehensive evaluation systems will be established. Combining the multiple advantages of dBECM, stem cells and novel preparation technology, the novel scaffold MSCs+dBECM-PGA+HA might be used to realize the safe and efficient repair for bone defect.