Exploiting crosslinked decellularized matrix to achieve uterus regeneration and construction
Exploiting crosslinked decellularized matrix to achieve uterus regeneration and construction
复制标题
利用交联脱细胞基质实现子宫再生和构建
DOI：
10.1080/21691401.2019.1699828
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发表时间：
2020-01
影响因子：
5.8
通讯作者：
Zhao Ying-Zheng
中科院分区：
文献类型：
--
作者：
Yao Qing;Zheng Ya-Wen;Lin Hui-Long;Lan Qing-Hua;Huang Zhi-Wei;Wang Li-Fen;Chen Rui;Xiao Jian;Kou Longfa;Xu He-Lin;Zhao Ying-Zheng
Abstract Decellularized extracellular matrix (dECM) has been considered as a promising scaffold in xenotransplantation, yet natural tissue dECM is often mechanically weak and rapidly degraded, compromising the outcomes. How to restore the mechanical strength and optimise the in vivo degradation, but maintain the microstructure and maximumly suppress the immune rejection, remains challenging. For this aim, we prepared and characterised various crosslinked decellularized rabbit uterus matrix (dUECM) and evaluated in vivo performance after uterus xenotransplantation from rabbit to rat. Naturally derived genipin (GP) and procyanidins (PC) were chosen to crosslink the dUECM, producing significant mechanical enhanced crosslinked-dUECM along with prolonged enzymatic degradation rate. Xenogeneic subcutaneous graft studies revealed that PC- and GP-crosslinked dUECM experienced significant cell infiltration and caused low immune reactions, indicating the desired biocompatibility. In vivo transplantation of GP- and PC-crosslinked dUECM to a uterus circular excised rat yielded excellent recellularization ability and promoted uterus regeneration after 90 days. While the reconstruction efficacy of crosslinked dUECM is highly depended on the crosslinking degree, crosslinking condition must be carefully evaluated to balance the role of crosslinked dECM in mechanical and biological support for tissue regeneration promotion.
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影响因子：
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作者：
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通讯作者：
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K. Schneider;M. Enayati;C. Grasl;I. Walter;L. Budinsky;Gabriel Zebic;C. Kaun;Anja Wagner;Klaus Kratochwill;H. Redl;A. Teuschl;B. Podesser;H. Bergmeister