治疗ED的一线药物对糖尿病勃起功能障碍（erectile dysfunction,ED）的疗效欠佳，寻找治疗糖尿病ED的方法十分必要。内皮功能障碍是糖尿病ED发生及发展的重要病因。研究发现eNOS增强剂AVE9488可明显增加eNOS产生及增加NO生物利用率，并能抑制内皮素的产生，改善内皮功能。miRNA126也能明显的增加内皮细胞NO生物利用率及内皮祖细胞修改内皮能力。纳米颗粒具有无毒、相容性好及增加药物的疗效而具有良好的应用前景。前期研究中已成功合成AVE9488-miRNA126-G5-PAMAM dendrimer纳米复合物并能改善高糖培养下阴茎内皮细胞中NO-cGMP通路。因此本课题拟将从体内及体外实验证实以上纳米复合物通过改善eNOS的表达，增强NO-cGMP通路；抑制内皮素的产生进而抑制RhOA/ROCK通路及增强内皮细胞修复能力改善勃起功能。为临床上治疗糖尿病ED奠定基础。

Diabetes patients with ED have poor response to the PDE5 inhibitors，so it is very necessary to find an effective treatment . It is well established that endothelial dysfunction play an important role in the development of diabetes ED. Several studies suggested that eNOS enhancer AVE9488 can enhance NO bioavailability and inhibit endothelin expression.miRNA126 can also improve NO bioavailability and improve the ability of endothelial progenitor cells repairing endothelium.Non-toxic,good biocompatibility nanocarrier which enhance drug effect show great promising applications. So we will prove that AVE9488-miRNA126-G5-PAMAM dendrimer improve diabetes ED through enhancement of NO bioavailability, inhibiting RhOA/ROCK pathway and improve the ability of endothelial progenitor cells repairing endothelium in vitro and in vivo which improve erectile function.The study will lay a foundation for treatment of diabetic ED.