光能转变为化学能是由叶绿体类囊体膜上两大色素蛋白复合物光系统I（PSI）和光系统II完成的。PSI利用光能催化电子从囊腔侧质体蓝素到基质侧铁氧还蛋白的跨膜传递，还参与调节光合作用对环境的适应。尽管PSI的结构解析已精确到原子水平，但是其生物发生及功能调节还不清楚。为发现新的PSI调控因子，我们用生化方法筛选与Pyg7互作的蛋白组分，发现未知功能蛋白PDP10（PSI Deficient Protein 10）的缺失导致植株生长弱小，PSI功能受损，对高光敏感。酵母双杂交实验表明PDP10与PsaF亚基有互作。PDP10包含DnaJ结构域，仅在陆地植物中存在。本项目拟通过对pdp10突变体的功能研究，揭示PDP10调控PSI生物发生和功能的机理,为提高光合作用的环境适应能力提供线索。

The conversion of sunlight into chemical energy is carried out by photosystem I (PSI) and photosystem II. PSI catalyzes the electron transfer from plastocyanin in the thylakoid lumenal side to ferredoxin in the thylakoid stroma side and plays important roles in the acclimation of photosynthesis to the changing environment. Although the structure of PSI has been resolved at atomic resolution, the regulation of its biogenesis and function is poorly understood. To identify novel factors involved in regulation of PSI biogenesis and function, we identified possible regulatory factors in the complex containing Pyg7 by biochemical immuno-affinity method and screened for PSI deficient mutant. Deletion of an unknown protein PDP10 (photosystem I deficient protein 10) leaded to a specific impairment in PSI function, retarded growth and more PSI sensitivity to high light. The PDP10 protein interacted with PsaF subunit in yeast. The PDP10 protein contains DnaJ-like zinc finger domain and is conserved in land plants. This study aims to characterize the function of PDP10 in the regulation of PSI biogenesis and function which will provide new insights in the acclimation of photosynthesis to environmental stress.