本项目以lncRNA为视角，沿着lncRNA TUG1/miR-339-3p/Rab7a构成ceRNA关系的思路，朝着系统阐述此调控模式借助脂类自噬，清除心肌脂毒性的目标展开工作。研究由体外细胞水平、体内动物实验和临床血清样本三个层面构成；体外实验采用H9C2细胞脂毒模型，运用双荧光素酶报告基因等技术证实三者间相互关系；使用基因过表达和基因回补等方法，通过检测分析脂类自噬和心肌脂毒性相关指标，评估清除心肌脂毒性的效率，系统阐述心肌脂毒性的清除机制。体内实验以糖尿病心肌病大鼠模型为对象，导入过表达TUG1载体，通过检测心肌脂毒性、自噬及心功能等指标，在体评价TUG1调控效率并证实此调控机制。临床样本验证以糖尿病心肌病、冠状动脉粥样硬化心脏病患者和健康成人为对象，检测血清中TUG1等指标，以临床样本验证此调控机制参与调节糖尿病心肌脂毒性损害，为诊断和治疗糖尿病心肌病提供新的思路。

To explore the mechanism and effect of lncRNA TUG1/miR-339-3p/Rab7a axis on cardiac lipotoxicity by lipophagy, the ceRNAs relationships between them will be verified by dual-luciferase reporter assay in vitro. In H9C2 cardiomyoblasts with palmitic acid and rats with diabetic cardiomyopathy, the effects of ceRNAs on cardiac lipotoxicity will be evaluated by overexpression of TUG1 gene and and complementation of the target gene. The serum levels of TUG1 will be detected among healthy population, patients with diabetic cardiomyopathy and coronary heart disease. The correlation between TUG1 level and cadiac function will be analysed for demonstrating the role of ceRNAs in diabetic cardiomyopathy. Our aim is to provide a new strategy for diagnosis and treatment of diabetic cardiomyopathy.