捕食线虫真菌是一类具有重要理论研究和应用价值的真菌，了解其捕食器官形成的分子机制是阐明其致病机理的关键。申请人前期发现捕食线虫真菌寡孢节丛孢捕食器官的形成可能受到长非编码RNAs(lncRNAs)的调控。而lncRNAs的转录丰度及稳定性受生物的RNA降解机制所控制。使RNA降解途径中的关键蛋白失活将放慢lncRNA的降解速度，不仅有利于发现更多隐性存在的lncRNAs，还有助于对其开展功能研究。基于此，本研究拟在前期研究的基础上，对寡孢节丛孢RNA降解途径关键蛋白敲除菌株进行高通量测序，以期发现更多参与捕食器官形成的ncRNAs，并选取2-3条具有潜在功能的lncRNAs开展分子生物学研究，揭示其在捕食器官形成过程中的分子调控机制，为从RNA调控角度理解真菌通过形态学改变完成侵染的致病机理奠定理论基础，为开发高效的线虫生防制剂提供理论指导。

Nematode-trapping fungi are a kind of microorganisms with important theoretical research and application value. Understanding the molecular mechanisms of their trap formation is the key to elucidate their pathogenesis mechanism. Our previous studies suggested that the trap formation of Arthrobotrys oligospora may be regulated by the long noncoding RNAs (lncRNAs). The transcript abundance and stability of lncRNAs are controlled by the RNA degradation mechanisms of species. Knockout/down of the functional protease encoding genes in RNA degradation pathway would slow the degradation of lncRNAs in cell. This method will not only help us to identify more lncRNAs that cannot to be easily found in wild type cell, but also raise the expression levels of lncRNAs, providing chances to study molecular mechanisms of these functional lncRNAs. In this study, we would like to use high-throughput sequence methods to discovery more cryptic ncRNAs involving in the trap formation. Moreover, the biological functions of 2-3 lncRNAs will be characterized through molecular biotechnologies, revealing their molecular regulation mechanism during trap formation. All these studies will provide a basis for elucidating the pathogenicity mechanism of fungi based on RNA regulation aspect and provide theoretical foundation for utilizing these fungi as high effective bio-control agents in the future.