真菌从腐生到寄生的生活方式转换是实现其侵染的关键，是生命科学领域有待加强研究的重要科学问题。捕食器官的形成是捕食线虫真菌实现对线虫侵染，从腐生转向寄生的标志。前期研究提示在捕食线虫真菌寡孢节丛孢中可能存在某些长非编码RNA（lncRNA）参与调控捕食器官的形成。本项目拟以寡孢节丛孢为研究对象，通过高通量测序技术对捕食器官形成过程中的几个关键节点进行测序，利用生物信息学手段筛选可能在寡孢节丛孢捕食器官产生过程中发挥功能的lncRNA，进而利用分子生物学手段对lncRNA进行实验验证及生物学功能研究，探明在寡孢节丛孢等捕食线虫真菌通过产生捕食器官实现从腐生向寄生生活转换过程中lncRNA的功能和调控机制，为理解真菌通过转换生活方式实现侵染的致病机理奠定理论基础，为开发有效的线虫生防产品提供理论指导。

The key of one fungus infects against its hosts is to switch its lifestyles from saprophytic to parasitic. This is the most important scientific question needs more studies in the future. The formation of trapping devices by nematode-trapping fungi is an important indicator of their switch from the saprophytic to the predacious lifestyles. Previous studies suggested that the formation of trap structures of Arthrobotrys oligospora maybe regulated by some long noncoding RNAs. In this study, the samples collected from the four key time points during trap formation of A. oligospora will be high throughput sequenced based on RNA-seq. The lncRNAs that might have functions in trap formation will be predicated through bioinformatic methods. Moreover, the biological functions of lncRNA will be characterized by using molecular biotechnologies. All these studies will help us from the perspective of long noncoding RNAs to understand the molecular mechanism of nematode-trapping fungi switching its lifestyles from saprophytic to predacious, also provide a basis for elucidating the pathogenicity mechanism of fungi and provide theoretical foundation for utilizing these fungi as high effective bio-control agents in the future.