甲基苯丙胺诱导雌性“高性动机”动物模型的神经环路机制解析

性动机异常是临床上女性性功能障碍的常见特征，但目前对性动机的神经生物学环路机制仍知之甚少。作为一种神经活动激动剂，甲基苯丙胺使用者处于一种“高性动机”状态。申请人的前期研究发现，前额叶皮层-内侧杏仁核投射环路参与并介导了甲基苯丙胺诱导的雌性动物的“高性动机”状态， 提示了前额叶皮层-内侧杏仁核投射环路在性动机的环路机制中的重要作用。本项目拟采用动物行为学、病毒环路标记、神经药理学、化学遗传学及电生理记录等技术方法，阐明前额叶皮层-内侧杏仁核投射环路在甲基苯丙胺介导的性动机增强中的作用，以期深化对性动机的神经环路机制的认识，为相关疾病的治疗提供新靶点新思路。

Abnormal sexual motivation is a common feature of female sexual dysfunction, but the neurobiological mechanism of sexual motivation is still poorly understood. As a central nervous system stimulant, methamphetamine users are in a state of "high sexual motivation". Our previous study found that the circuit of mPFC-AMG participated in and mediated the "high sexual motivation" state of female animals induced by methamphetamine, suggesting that the mPFC-AMG plays an important role in the circuit mechanism of sexual motivation. In this project, behaviroural test, neural circuit tracing, neuropharmacology, chemical genetics and electrophysiological recording were used to elucidate the role of mPFC-AMG circuit in methamphetamine mediated sexual motivation enhancement. Overall, the goal of this study is to deepen the understanding of the circuit mechanism of sexual motivation and provide new targets and mechanisms for the treatment of related diseases.