干细胞移植和治疗性血管生成已成为心肌梗死后心肌重建的研究热点，但梗死后缺血微环境却不利于移植干细胞的归巢、分化和增殖，微环境重建成为决定干细胞心脏移植疗效的关键。Angiopoietin-1可促使血管生成和成熟，促使梗死心肌血运结构重建，缺血时却明显下调，通络方剂通心络可疏通血络，促使血运功能重建，两者均可改善移植微环境，并促使移植干细胞存活和分化。课题拟以编码Ang-1基因腺病毒载体转染自体MSCs经冠脉移植小型猪心肌梗死区域，通过表达Ang-1并联合通心络治疗，"里应外合"以促进梗死心肌血运结构和功能重建，改善移植微环境并促使移植干细胞归巢、分化和增殖，促进心肌梗死后心肌重建和功能恢复，并观察Ang-1和通心络对MSCs迁移，分化以及SDF-1、CXCR-4、VCAM-1、SOD等表达的影响以探讨其改善移植微环境的机制。课题将为中西医结合基础上的干细胞心脏移植以及移植微环境改造提供依据

Stem cells transplanting and therapeutic angiogenesis have been the hot research areas of myocardial revascularization after myocardial infarction. But it is harmful for stem cells to homing, differentiation and multiplicationin the ischemic microenvironment. The low survival rate of stem cells in the ischemic myocardium microenvironment is the bottle neck in treating ischemic diseases with stem cell transplantation .So the microenvironment remolding becomes the key to determine the therapeutic effect of stem cell transplantation after myocardial infarction. Angiopoietin-1 can not only promote angiogenesis and blood vessel maturity, but also improve the structure reconstruction of blood circulation after myocardium infarct. However expression of angiopoietin-1 was down regulated in the ischemic myocardium. Meanwhile, Tongluo prescription - tongxinluo capsule can dredge the blood vessel and improve microcirculation to promote the functional recovery of of blood circulation. Both of them can improve the transplanting microenvironment and increase the chances of survival of the transplanting stem cells survive and differentiation. The study is to use adenovirus carrier of coding angiopoietin-1 gene to transfect autologous mesenchymal stem cell for transplantation into the infarcted myocardium in miniature pig via coronary artery. Expression of angiopoietin-1 and the treatment of tongxinluo capsule will promote the structure reconstruction and functional recovery of blood circulation in infarcted myocardium, and improve the transplanting microenvironment and motivate homing, differentiation and proliferation of the transplanting stem cells, and enhance the structure reconstruction and function recovery of myocardium after myocardial infarction. This Lab will observe the effect of angiopoietin-1 and tongxinluo capsule on the migration and differentiation of MSCs and the expression of SDF-1, CXCR-4, VCAM-1, SOD et al, which can probe into its mechanism of improving the transplanting microenvironment. The study will provide the basis for stem cells transplantation and remolding of transplanting microenvironment after myocardium infarct on the basis of integrated traditional and western medicine,as a whole and local treatment.