新皮质癫痫的致痫区可能具有网络特征，常规方法较难准确定位。近年的研究表明，颅内脑电图中的高频振荡（HFO）与癫痫的形成和发作密切相关，癫痫患者脑内的HFO还可能帮助定位致痫区，指导癫痫手术。但是，目前HFO的研究一般基于颞叶内侧面癫痫，HFO在新皮质癫痫中的分布、作用、与致痫区的关系，以及产生机制都还有待进一步研究。申请人在前期研究中发现癫痫患者大脑新皮质中的病理性HFO (pHFO)与癫痫发生紧密联系，而且pHFO的分布还具有多灶性特点，pHFO灶点之间可能通过相互联系和相互作用导致癫痫发作。课题组拟在临床研究和整体动物模型两个层次上，以常规电极、微电极和微电极阵列的多维度记录手段，阐明pHFO和pHFO网络在新皮质癫痫发生中的作用，并明确pHFO产生的细胞机制。本项目不仅能加深对新皮质癫痫发生机制和致痫区网络学说的认识，还能为临床致痫区定位提供指导。

The epileptogenic zone in neocortical epilepsy can be a complicated network, which is difficult to be localized by routine methods. High frequency oscillation (HFO), a tiny EEG signal recorded by intracranial EEG, is closely related to seizure generation. More recently, HFOs are found to be closely related to epileptogenic zone in clinic and provide useful information in making the surgical plan. Current HFO studies were usually based on mesial temporal epilepsy. However,in neocortical epilepsy, the distribution, role in seizure generation, relationship with epileptogenic zone as well as the cellular mechanism of HFO remains unknown. We have explored the HFO distribution in patients with neocortical epilepsy and found that both physiological HFOs and pathological HFO (pHFO) exist in human neocortex. Furthermore, the pHFOs were multi-focally distributed; and the pHFO foci may be inter-connected and the interaction may participate ictogenesis. Here we plan to study the role of pHFO and pHFO network in seizure generation of neocortical epilepsy in clinical level as well as in animal epilepsy model using a combination of macro-electrode and micro-electrode. We also plan to elucidate the cellular mechanism of neocortical pHFO generation using microelectrode array. Our project will help to understand neocortical epilepsy generation and epileptogenic network; it can also promotes pHFO application in localizing epileptogenic zone in neocortical epilepsy.