胆囊癌预后极差，导致的死亡绝大部分是由于的肿瘤复发转移造成。因此，针对其发病和复发转移机制研究具有重要意义。有研究报道lncRNA与多种类型的肿瘤发生发展密切相关，但lncRNA对胆囊癌的调控机制报道较少。MALAT1是相关联基因数最多的lncRNA 之一，它在胆囊癌细胞中具有调控增殖、凋亡、转移的重要生物学功能。同时miR-31参与介导胆囊癌细胞的发生发展过程。我们前期工作确定了MALAT1 在胆囊癌中高表达，并且参与胆囊癌的发生，还鉴定了MALAT1 对miR-31具有负调控的作用。在此基础上，本课题将深入探究 MALAT1 促进胆囊癌发生发展的分子机制。研究其与miR-31相互作用机制与生物学功能，从而明确 MALAT1 作为胆囊癌诊治新靶点的临床意义。

The prognosis of gallbladder carcinoma is really poor and its cause of death is mainly due to tumor recurrence and metastasis. Therefore, understanding the pathogenesis and mechanism of tumor recurrence and metastasis has great impact on the early diagnosis and better treatment outcome. Studies have reported that lncRNAs are involved in the carcinogenesis of many types of cancers including liver and breast cancers. However, the regulatory mechanism of lncRNA in the oncogenesis of gallbladder carcinoma was rarely known. MALAT1 is one of the lncRNAs which is associated with the regulatory pathways of various genes. Our preliminary studies indicated that MALAT1 is highly expressed in gallbladder carcinoma, and it exhibits important biological functions in the regulation of proliferation, apoptosis and metastasis in gallbladder carcinoma. At the same time, we also identified that miR-31 is involved in the occurrence and development of gallbladder cancer cell. Also, we have found that MALAT1 has a negative regulatory effect on miR-31. Based on our results, we propose to further explore the detailed molecular mechanism of how MALAT1 promotes the occurrence and development of gallbladder carcinoma. We will also study the mechanism of how MALAT1 interacts with and regulates miR-31 as well as its biological functions. Our study could help us pinpoint novel biomarker for the early diagnosis and indentify potential treatment target for gallbladder carcinoma.