病毒病是影响大白菜生产的主要病害，芜菁花叶病毒（TuMV）是引起该病害的主要病原。eIF(iso)4E一类非常重要的TuMV抗性基因，在TuMV侵染植物寄主时与VPg蛋白互作，起始病毒的翻译、增殖。白菜基因组中存在3个eIF(iso)4E拷贝，TuMV中存在C1~C5 株系。目前在十字花科芸薹属中还没有报道eIF(iso)4E与TuMV抗性的系统研究。本项目拟对105份白菜材料eIF(iso)4E进行变异分析，同时对TuMV C1~C5 VPg进行变异分析。通过酵母双杂实验，系统研究白菜eIF(iso)4E 3个拷贝与5种TuMV株系VPg的互作关系。结合eIF(iso)4E和VPg变异分析结果，明确在eIF(iso)4E与VPg互作中，起作用的eIF(iso)4E差异区段和TuMV VPg差异位点，揭示白菜eIF(iso)4E对TuMV的抗性机制，为大白菜抗TuMV分子育种提供理论基础。

The virus disease is the main disease affecting the production of Chinese Cabbage. Turnip mosaic virus (TuMV) is the main pathogen causing virus disease. eIF(iso)4E is a kind of very important resistence gene which can interact with protain VPg and initiate the translation and propagation of the virus when TuMV infects the plant host. There are three eIF(iso)4E copies in the genomes of Brassica rapa. And there are C1~C5 TuMV striains. No researches about eIF(iso)4E and TuMV resistance have been reported in Cruciferae brassica species so far. This project aims to analyse the variation of eIF(iso)4E in 105 Brassica rapa varieties and the variation of TuMV C1~C5 VPg. Yeast Two-Hybrid experiement is conducted to study the interacton between three copies of eIF(iso)4E in Brassica Rapa and VPg in five TuMV strains. Combined With the variation analysis of eIF(iso)4E and VPg, we confirm the functional differentiated regions and amino acid sites in the interaction of eIF(iso)4E and VPg- - thus clarify the molecular mechanisms of eIF(iso)4E gene's resistance against TuMV and lay a theoretical foundation for the molecular breeding of Chinese cabbage with TuMV resistance.