Development of gene-based markers for the Turnip mosaic virus resistance gene retr02 in Brassica rapa
Development of gene-based markers for the Turnip mosaic virus resistance gene retr02 in Brassica rapa
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芜菁花叶病毒抗性基因 retr02 基因标记的开发
DOI：
10.1111/pbr.12372
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发表时间：
2016-08
期刊：
影响因子：
2
通讯作者：
Sun Ri-Fei
中科院分区：
文献类型：
--
作者：
Li Guo-Liang;Qian Wei;Zhang Shu-Jiang;Zhang Shi-Fan;Li Fei;Zhang Hui;Wu Jian;Wang Xiao-Wu;Sun Ri-Fei
芜菁花叶病毒（Turnipmosaicvirus，TuMV）是影响大白菜生产的一种严重病害。前期的研究已经克隆了一系列TuMV抗性基因并开发了分子标记。本研究基于大白菜抗TuMV的单隐性基因retr 02，通过在外显子1和内含子1的连接处增加一个G，开发了一个衍生的切割扩增多态性序列（dCAPS）标记和一个竞争等位基因特异性PCR（KASP）标记。这两个标记能够在大白菜育种中检测到retr 02等位基因。与dCAPS标记相比，KASP标记具有灵活性、成本效益和快速处理的优点，这可能有利于建立用于标记辅助选择的高通量检测方法。
Turnip mosaic virus (TuMV) is responsible for a serious disease that affects the production of Chinese cabbage. Previous studies have cloned a series of TuMV resistance genes and developed molecular markers. In this study, a derived cleaved amplified polymorphism sequence (dCAPS) marker and a Kompetitive Allele Specific PCR (KASP) marker were developed based on a single recessive gene, retr02, which confers broad-spectrum TuMV resistance in Chinese cabbage by means of an additional G at the junction of exon 1 and intron 1. The two markers were able to detect the retr02 allele in Chinese cabbage accessions used in breeding programmes. Compared with the dCAPS marker, the KASP marker was flexible, cost-effective and quick to process, which is likely to be beneficial in establishing high-throughput assays for marker-assisted selection.
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影响因子：
4.4
作者：
Wang Y;Sun S;Liu B;Wang H;Deng J;Liao Y;Wang Q;Cheng F;Wang X;Wu J
通讯作者：
Wu J
影响因子：
3.1
作者：
Wenxing Pang;Xiaonan Li;S. Choi;Van Dan Nguyen;Vignesh Dhandapani;Yoon-young Kim;N. Ramchiary;
通讯作者：
Wenxing Pang;Xiaonan Li;S. Choi;Van Dan Nguyen;Vignesh Dhandapani;Yoon-young Kim;N. Ramchiary;
影响因子：
3.5
作者：
Jenner, CE;Wang, XW;Walsh, JA
通讯作者：
Walsh, JA
影响因子：
3.8
作者：
Rusholme, Rachel L.;Higgins, Erin E.;Lydiate, Derek J.
通讯作者：
Lydiate, Derek J.
影响因子：
3.1
作者：
William L. Holdsworth;M. Mazourek
通讯作者：
William L. Holdsworth;M. Mazourek