目前关于低温促进大豆疫霉游动孢子释放的分子机理尚无人研究。本项目以低温处理的大豆疫霉孢子囊为研究对象，利用数字基因表达谱（digital gene expression profile，DGE）测序技术，分析经低温诱导后孢子囊的差异表达mRNA标签；同时，以高通量（454 GS FLX）测序孢子囊样品全转录组信息为对照，通过DGE 与454 GS FLX 序列数据的比对分析，获得差异基因表达谱；采用实时定量PCR 验证与分析差异表达基因的表达特征及可能参与的信号通路， 并作为候选差异表达基因，利用RACE 技术获得其基因全长cDNA。该项目的研究结果将揭示低温诱导大豆疫霉游动孢子释放的分子机理，并为药剂防治大豆疫霉提供新的靶标位点，为可持续控制大豆疫病提供理论基础。

The molecular mechanism, which low temperature can promote the zoospores of Phytophthora sojae to release has not been studied. Sporangia of Phytophthora sojae induced by low temperature as materials in this project, and differential expressed gene mRNA tags induced by low temperature in sporangia will be generated by digital gene expression profiling (DGE) analysis. Additionally, high-throughput sequencing (454 GS FLX) will also applied to generate whole transcriptome profiling of the sporangia. By comparing data generated by DGE and that of 454 GS FLX, differential expressed genes and networks will be analyzed. Special expression character of the genes and signal pathways they may be involved will be revealed and confirmed by real-time quantitative PCR. Candidate differential expressed genes will be picked out based on these data, and the full length cDNAs will be synthesized by RACE technology. The results of these studies will not only revealed the molecular mechanism which low temperature can promote the zoospores of Phytophthora sojae to release, but also provide a new target sites for chemical control of Phytophthora sojae and a theoretical basis for the sustainable control of soybean blight.