砷 (arsenic, As) 污染已成为全球性的环境问题，As污染对人体健康的危害也引起人们的高度关注。近年来研究发现As不仅能够诱导机体发生病变，而且对机体的内分泌系统也有干扰效应。现有研究指出As对甲状腺内分泌系统的干扰可能是砷诱导一些相关疾病发生的主要原因。确定As对甲状腺内分泌系统干扰机制是诊断和治疗一些与As通过干扰甲状腺内分泌系统引起的相关疾病的基础。本项目以非洲爪蟾变态发育时期（蝌蚪）为实验材料，选择两种常见的无机砷：AsV和AsIII，在测定其对蝌蚪体内甲状腺激素含量的干扰和在蝌蚪体内富集情况的基础上，通过转录组学筛选砷干扰甲状腺内分泌系统敏感靶基因，qPCR再次验证的方法探究AsV和AsIII对甲状腺内分泌系统的干扰机制。这些工作将为全面系统地评价As的内分泌毒性提供基础数据，并为诊断和治疗As通过干扰甲状腺内分泌系统导致的一些疾病提供治疗依据。

Arsenic (As) pollution has becoming the global issue, and As toxicity has been attracting more attentions from public. In recently, some studies found that As not only induced pathological changes, but also disrupted the endocrine system. There some researches proposed that the disruption of As on thyroid endocrine system could be the main reason for some illnesses caused by As. Understanding the interference mechanism of As on thyroid endocrine system is important to diagnose and cure the illnesses caused by As via disrupting on thyroid endocrine system. In this study, xenopus laevis will be emplyed to analyze the interference mechanism of arsenic (AsV and AsIII) on thyroid endocrine system via integrating transcriptome and qPCR verification, after measuring the thyroid hormone levels and total As contents in xenopus laevis. Comprehensive understand the mechanism of As disruption on thyroid endocrine via integrating transcriptome and metabolomics. In order to provide the evidences for diagnosing and curing the illnesses caused by As via disrupting on thyroid endocrine system.