利用蛋白质组学和生物信息学的研究策略，筛选和鉴别与家蚕胚胎滞育和滞育解除有关的功能蛋白，克隆相关的基因，查明其作用功能。试验将从滞育发动初期、滞育维持阶段和滞育解除后等时期获得家蚕胚胎总蛋白质，采用液相色谱串联质谱联用(LC-MS/MS)的技术方法鉴定各时期蛋白质种类，通过差异比对，鉴别分析，蛋白质数据库查询和生物信息学分析，得到与滞育和滞育解除相关的关键蛋白，研究其生物学性质，探究它在滞育发动、滞育解除过程中的时序变化、差异表达，查明蛋白质的相关功能。根据候选蛋白质的氨基酸序列预测其核苷酸序列，设计引物，通过基因工程的技术方法，克隆获得调控滞育和滞育解除相关的候选基因，利用实时荧光定量PCR与Western blot的方法，分析其在滞育发生和滞育解除中的差异表达，mRNA表达水平与滞育强度的联动效应，探索其功能作用，从分子水平的角度进一步阐明家蚕滞育及其解除的机理。

With the analytic strategies of proteomics and bioinformatics, the relevant proteins of diapause origination and termination are attempted to be screened to identified from embryo in Bombyx mori. These genes will be cloned and their function will be analysed. The total proteins will be extracted from the embryo during the stage of diapause origination or diapause standing of diapause termination. By the method of LC-MS/MS, the protein composition each stage will be identified. Through difference comparison, identified analysis, querying in protein database and bioinformatics analysis, the relevant proteins of diapause origination and diapause termination will be obtained. Therefore, their biological properties, temporal variation, differential expression will be determined. According to the nucleotide sequence deduced from the amino acid sequence of candidate proteins, the primers will be designed and the genes related with diapause origination and diapause termination. With the methods of real-time quantitative PCR and Western blot, differential expression of these genes will be analysed during diapause origination and diapause termination of embryo in Bombyx mori. Their function and connected effect between diapause degree and mRNA expression level will be investigated. These result will elucidated furtherly the mechanism about embryo diapause and diapause termination in molecullar level in Bombyx mori.