上皮-间质转化(EMT)是肺腺癌细胞转移的关键途径。本课题组前期研究首次发现miR-19具有促进肺腺癌细胞EMT作用，其机制可能与抑制miR-19/PTEN信号轴有关；六神丸具有抑制肺腺癌转移、肺腺癌细胞EMT以及miR-19/PTEN信号轴作用。基于miR-19/PTEN信号轴的病理学特性和本课题组的前期研究成果我们提出假说：六神丸通过作用于miR-19/PTEN信号轴抑制肺腺癌细胞EMT。本课题中我们将从细胞和动物水平，采用分子生物学、小动物活体内成像技术对miR-19/PTEN信号轴在六神丸抑制肺腺癌细胞EMT中的作用进行研究。本项目将有助于深入阐明肺腺癌转移机制，发掘肺腺癌转移的治疗靶点，丰富中药治疗的疗效机理，为肺腺癌转移的防治探索一条新的思路和途径。

Epithelial-mesenchymal transition (EMT) is a key process in the metastasis of lung adenocarcinoma cells. In our preliminary studies，we found that miR-19 promote EMT in lung adenocarcinoma cells for the first time, and its mechanism may be related to the inhibition of miR-19/PTEN signal axis. Liushen pills can inhibit the metastasis of lung adenocarcinoma, EMT in lung adenocarcinoma and miR-19/PTEN signal axis. Based on the pathological characteristics of miR-19/PTEN signal axis and our preliminary studies, we hypothesize that Liushen pills inhibit EMT in lung adenocarcinoma cells by acting on miR-19/PTEN signal axis.In this project, molecular biological technology and small animal in vivo imaging technology are taken to investigate the role of miR-19/PTEN signal axis in Liushen pills inhibiting EMT in lung adenocarcinoma cells from cellular and animal levels.This project aims to further elucidate the mechanisms of lung adenocarcinoma metastasis, seek therapeutic targets for the metastasis of the lung adenocarcinoma, enrich the mechanisms of traditional Chinese medicine anti-lung adenocarcinoma metastasis, and thus to discover a new way for prevention and treatment of lung adenocarcinoma metastasis.