水稻气孔开关控制基因ROC8的图位克隆与功能解析
结题报告
批准号:
32001532
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
崔学安
学科分类:
作物基因组及遗传学
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
崔学安
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中文摘要
气孔是植物与外界环境进行物质交换的主要通道。气孔能快速地感知外界信号,并通过其开放或关闭程度影响植物的光合作用、蒸腾作用、逆境胁迫等重要的植物生化路径,关系到作物的水分消耗和产量形成。陆生植物在干旱条件下,一般通过关闭气孔来降低蒸腾系数,抑制水分丧失从而提高植物抵御干旱的能力,但目前对水稻气孔开闭而影响其耐旱的机理研究甚少。本课题中,我们从水稻突变体库筛选获得一份叶片持续萎焉的突变体Cwm1,受一对显性核基因控制,光合参数测定和电镜分析表明突变体Cwm1的气孔一直开放是造成叶片萎焉的主要原因。图位克隆该基因,其编码转录因子ROC8,过表达ROC8基因重现了突变体表型。为进一步研究ROC8基因参与调控水稻气孔开闭的作用机理,本项目拟通过植物生理学、生物化学、分子遗传学等手段分析ROC8基因的功能,寻找与ROC8相互作用的蛋白以及其调控的下游靶基因,为培育节水抗旱的水稻新材料提供分子基础
英文摘要
Stoma is one of the main exchange channels between plants and external environment. It can perceive external signal and then affects plant photosynthesis, transpiration, and abiotic stress through its opening or close, thus influencing grain yield. While the molecular mechanism regulating stomatal opening or close in rice is poorly understood so far. To identify the related regulators, we screened rice mutant population and obtained a constitutively wilted mutant (Cwm1), which was controlled by a single dominant nuclear gene. The photosynthetic parameter measurements and electron microscopy analysis results showed that the stoma of the cwm1 leaf keeps open during daytime and night. We isolated Cwml gene by map-based cloning, and it encodes a transcription factor ROC8 with unknown function. Over-expression of ROC8 in wild-type recovered the similar cwm1 mutant phenotype. In this project, we apply molecular biology, biochemistry and genetics approaches to further understand the molecular mechanism of ROC8 in controlling stoma opening and closing, exploring downstream targets and interaction partners of ROC8 during stoma opening and close will provide new molecular basis for drought-resistent rice breeding.
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DOI:10.1016/j.cj.2024.01.010
发表时间:2024-04-01
期刊:CROP JOURNAL
影响因子:6.6
作者:Wu,Suting;Kyaw,Htin;Cui,Xue'an
通讯作者:Cui,Xue'an
DOI:10.3389/fpls.2022.905111
发表时间:2022
期刊:Frontiers in plant science
影响因子:5.6
作者:
通讯作者:
国内基金
海外基金