牙釉蛋白在牙釉质形成中起到了关键作用，了解其作用机制有利于人工牙釉的合成。.牙釉蛋白在溶液状态下属于一种没有固定结构的蛋白，但会和牙釉材料表面结合。.结合后的蛋白会有一定的结构。牙釉蛋白的序列具有多样性，在牙釉形成过程中牙.釉蛋白的组分会有变化。由于牙釉蛋白的复杂多样性，同时缺乏合适的研究手段，.人们目前为止还没有功能状态下牙釉蛋白的高分辨率结构。本研究拟用固态核磁.方法，结合优化的样品制备方法，解析不同牙釉蛋白在和牙釉固体材料结合状态.下的结构。实验将从小的牙釉蛋白入手，由简入繁的逐步深入研究。本课题的最.终目标是获得多个牙釉蛋白高分辨率结构，了解不同牙釉蛋白结构的差异，从而.理解牙釉蛋白调控牙釉形成的机制。通过本课题的研究，我们有希望获得一套固.态核磁解析此类吸附蛋白结构的方法，为其它的类似的研究打下基础。

Amelogenin plays a key role in regulation of enamel formation. Amelogenin is an intrinsic disordered protein that does not adopt a specific structure in solution.However, it adopts certain conformations upon binding to enamel. Amelogenin is a group of proteins of different length. During the development of enamel, the length of amelogenin protein decreases, suggesting a regulation role. So far there is no high-resolution structure reported in its functional form. In this research, solid-state NMR techniques will be applied to study the structure of amelogenin with different lengths. The sample preparation will be optimized. The study will start with the smallest amelogenin. The high-resolution NMR structure obtained in this research will be helpful for us to understand the function of amelogenin in enamel formation. The research will also help us to develop a set of solid-state NMR tools to study similar surface binding proteins.