基于LDH/rGO/α-Fe2O3分体式光电免疫传感器的构建及肿瘤标志物检测

肿瘤标志物检测对肿瘤早期诊断具有重要的临床意义，寻找一种具有高灵敏、低干扰的免疫分析方法是实现癌症早期诊疗的关键。分体式光电免疫传感器的构建为提高肿瘤标志物检测灵敏度和抗干扰能力提供了可能的路径。本项目拟采用具有良好可见光响应α-Fe2O3作为吸光材料，引入导电性良好的rGO作为界面电荷传输层，并以LDH作为空穴捕获层和催化活性位点构建光电分离效率高的LDH/rGO/α-Fe2O3复合光电极。依据LDH层间和电子结构特点，结合磁分离技术和酶信号放大策略，组装葡萄糖氧化酶标记的磁珠夹心免疫复合物，据此构建具有抗干扰能力的光电免疫传感器。项目将围绕LDH/rGO/α-Fe2O3光电极的构建及其性能的优化，重点研究揭示LDH与rGO协同增强α-Fe2O3光电分离效率机制，探索LDH形貌及电子结构与选择性反应的构效关系，最终建立一种高灵敏度和低干扰检测免疫检测平台。

Tumor marker detective technology is almost the only non-invasive effective way to find out malignant tumor in the early diagnosis. Tumor marker detective technology is an effective non-invasive way to find out malignant tumor in the early diagnosis. Developing of immunoassay technology with high-sensitivity;and low-interference is crucial for the detection of tumor markers. Construction of split-type photoelectrochemical (PEC) immunosensor is a promising way to improve the detection sensitivity and anti-interference ability of tumor markers. In which, LDH/rGO/α-Fe2O3 composite photoanode with a high photoelectric conversion efficiency will be constructed by using α-Fe2O3 with good visible light response as the light-absorbing material, rGO with excellent conductivity as the interface charge transfer layer and LDH nanosheets as the hole capture layer, respectively; meanwhile, according to the lamellar and electronic structure structures of LDH, combined with magnetic separation technology and enzyme signal amplification strategy, a magnetic bead sandwich immune complex labeled with glucose oxidase will be assembled as biometric unit. In general, the project focuses on the creation and performance optimization of high performance LDH/rGO/α-Fe2O3 based PEC immunosensor. At the same time, we will study and reveal the synergetic mechanism for the enhanced charge separation efficiency of α-Fe2O3, and the structure-activity relationship between the morphology and electronic structure of LDH and the reaction selectivity. In the end, a new and efficient tumor marker diagnosis platform will be built after exploring the construction mechanism of the split-type PEC immunosensor.