荧光印迹阵列传感技术较传统快速检测技术在多靶标分析中显示出独特的优势，但是由于荧光单体或竞争吸附指示剂难寻，荧光印迹阵列传感器的构建仍然存在巨大挑战。本项目拟以氨基糖苷类抗生素为研究对象，首次提出基于印迹位点-比率型荧光探针双识别竞争吸附剂的传感新策略，在此基础上，发展一类比率型荧光印迹阵列传感器；探明传感体系对氨基糖苷类抗生素的响应规律，并开发复杂食品中多种氨基糖苷类抗生素残留同步检测的新方法。本项目研究的意义在于原创性地将分子印迹特异性识别、比率型荧光检测、阵列高通量分析三大优势相结合用于兽药残留分析领域，为开发快速检测技术开辟新的研究思路。

Compared with traditional rapid detection techniques, fluorescent imprinted array sensor exhibits unique superiority in analysis of multi-targets. However, the construction of fluorescent imprinted array sensor still remains a great challenge due to limited number of fluorescent monomers and competitive adsorption indicator. In this project, a new fluorescent response strategy for imprinted recognition of aminoglycosides based on bi-recognition competitive adsorbent for imprinted sites and ratiometric fluorescent probe is proposed for the first time. As a result, a novel ratiometric fluorescent imprinted array sensor will be developed. Additionally, the response rule of the fluorescent system will be comprehensively investigated. Furthermore, a new method for rapid detection of multiple aminoglycosides in complex food samples will be achieved. In summary, the project integrates the advantages of specific imprinted recognition, ratiometric fluorescent probe, and array analysis for detection of veterinary drugs, providing a new research platform for developing rapid detection technique.