通过分子设计,将具有荧光光学性能优异的双光子吸收和非线性光学等光敏基团，以及高选择性的探针识别基团，采用高效点击化学等方法，将他们分别组装到高热稳定并具有纳米大小的多面体笼状硅氧烷（POSS）无机材料中，有效克服凝聚态光学聚集效应，制备出灵敏度高、选择性好、不同结构与分子构筑的新型纳米无机杂化探针和生物传感功能材料；通过改变不同组分的结构，实现杂化分子结构的有效构筑，系统研究材料结构控制方法、影响因素与形成机理，采用4f相位相干成像和显微共聚焦、荧光显微以及荧光光谱仪等测试方法，研究材料的探针性能与机理、生物体内外材料的探针与传感效应，以及材料探针性能的聚集态增强机理，采用理论模拟和实验技术相结合,深入探讨杂化材料分子组成、微观与宏观结构与杂化材料分子探针与生物传感性能之间的关系与影响规律，为材料在环境与生物领域的应用与开发奠定基础。

The nano-sized inorganic hybrid functional materials as probes and biosensor with high sensitivity and well selectivity, were prepared based on molecular design and "Click Chemistry" method by incorporating a receptor with high selectivity and a fluorophore with two-photon absorption or nonlinear optical properties and so forth into the inorganic polyhedral oligomeric silsesquioxane for improving the sensitivity in condensed state condition, in which the nano-meter POSS were uniform dispersed to inorganic hybrid probes and biosensor materials. Through changing the structure of the different components, to achieve effective structural architecture of hybrid materials, the control method of materials synthesis, influence factors and formation mechanism were investigated in detail. The probe performance and mechanism, in vitro and/or in vivo biosensor effect, and aggregation induced luminescence enhancement effect (AIE) were systematically investigated and evaluated by 4f phase coherent imaging technique, confocal laser system with femtosecond pulse, fluorescence microscope, UV-vis and fluorescence spectral techniques. By combining the theory of simulation and experiment, the influence of molecular structure on properties of probe and relationship between molecular composition, micro and macro molecular structure and their biosensor performance of hybrid materials were investigated in detail for the application and development in the field of environmental and biological materials.