丹参制剂品种多样，导致生产中对药材内不同种类药效成分含量的要求存在差异。针对不同活性成分，开展定向代谢调控与育种，符合丹参的产业需求。转录调控因子是开展高效代谢调控的强大工具。目前，丹参中活性成分关联转录因子的发现主要基于信号分子的响应机制研究，多具广泛的代谢调控作用，不适合作为特定化合物的特异调控靶点。中药活性成分的合成和累积在其生长发育过程中呈现动态的变化，同时在不同器官、组织中特异合成和积累。如能基于活性成分合成途径的“时-空”特异性，阐明其转录调控机制，则可有效挖掘特定活性成分的关联调控因子。本项目拟在前期对丹参完整生长周期开展深入的转录研究的基础上，进一步开展高分辨率原位表达谱分析，解析丹参活性成分“空间”累积特异性的机制；整合“时-空”两个维度的信息，阐明关联机制，并筛选2-3个特异调控因子开展功能研究；进而开展特定活性成分的代谢调控。本研究将为开展丹参的定向育种奠定基础。

According to different requirements for main active components in different kind of Salvia miltiorrhiza preparations, there are variant demands for the content of diverse active compounds in this herb. Therefore, the industrial chain of S. miltirorrhiza requires the studies on breeding of S. miltirorrhiza to produce different varieties that highly produce specific active compounds. Transcriptional regulating factor (TF) is a powerful tool for metabolism regulating. In current, the discovery of TFs in S. miltirorrhiza mainly focus on the reprogramming of transcriptome that induced by plant hormones. However, most of the correlated TFs play wide range regulation roles on plant metabolisms. They are not the most suitable genes for targeted regulating of a specific metabolite. The metabolic flux in plants always presents dynamic changes during the whole life cycle. Meanwhile, the organs and tissues accumulation patterns are exist for most of metabolites. There are specific regulating mechanisms to control such “temporal and spatial” dynamic profiling of metabolisms. Therefore, exploring the transcriptional regulating mechanism would help to identify the specific TFs that control the specific metabolites. In previous study, we have studied the temporal transcriptional profiling during the whole lifecycle of S. miltirorrhiza, and predicted a series of correlated TFs. In this project, the in-situ transcriptional profiling will be carried based the laser capture microdissection (LCM) method, which will produced high resolution transcriptional profiles. To combine the “temporal and spatial” profiling, the unique TFs that control specific metabolites will be discovered. The regulating mechanism of these TFs will be explored by screening there target genes. Next, the target regulation of specific active compounds in S. miltirorrhiza will be performed in transgenic plant strains. This study will provide theoretical basis for the following targeted breeding of S. miltirorrhiza.