骨髓间充质干细胞（BMSCs）是肺癌等组织微环境中重要细胞之一，探明其遗传稳定性及其与肿瘤干细胞关联性对肿瘤防治研究具有重要意义。黄芪具有益气生精、扶正固本作用，BMSCs是骨髓精气在细胞层面的体现，课题前期研究证实黄芪可显著提高肺癌细胞微环境中BMSCs遗传稳定性。本项目在前期ras基因突变诱发线虫癌变的基础上，以ras基因转染BMSCs，并与肺上皮细胞共培养，利用FCM术、CCK8、激光共聚焦法等检测BMSCs细胞骨架形态、增殖性；采用RT-PCR、免疫组化及Western blot等技术检测Occludin和CK-18等肺上皮细胞标记物、CD133和CD44等肿瘤干细胞标记物、及ras和myc等基因及蛋白的表达；观察裸鼠实验分化细胞的成瘤性；并进一步利用黄芪干预观察对上述指标的影响；以期为BMSCs的ras突变与肺癌及其肺癌干细胞关联性，以及黄芪通过扶正固本防治肿瘤提供科学依据。

Bone marrow mesenchymal stem cells (BMSCs) is one of the important cell such as lung cancer microenvironment, it is a great significance to prove its genetic stability and its relevance to tumor stem cells for the prevention and treatment of cancer. Radix Astragali has the function of supplementing qi and nourishing essence, supporting healthy energy, BMSCs is the embodiment of the bone marrow nutrients at the cellular level. In previous studies, the genetic stability of BMSCs in lung cancer microenvironment was significantly increased by Radix Astragali. It is based on carcinogenesis induced by ras gene mutation in C. elegans and will set up a co-culture model of typeⅱalveolar epithelial cells ( AEC) and BMSCs transfected by ras gene. Using FCM、CCK-8、and confocal microscopy to detect cytoskeleton morphology and proliferation of BMSCs; Using RT-PCR、immunohistochemistry and western blot to test Occludin and CK-18 on AEC、CD133 and CD44 on lung cancer stem cells、ras and myc gene in lung cancer cells; The formation of tumor cells in nude mice was observed; The effects of cytoskeleton morphology and proliferation of Radix Astragali on BMSCs were also tested. That will provide a scientific basis for the prevention and treatment of tumors in Radix Astragali, the ras mutation of BMSCs and the association of lung cancer and lung cancer stem cells.