由于对人类早期造血发生的分子调控机制尚缺乏了解，急需建立一种理想的实验体系。我们报道了体外大量扩增hESC/hiPSC来源的造血干／祖细胞的方法，并证明了hESC可以诱导产生成体造血来源的功能成熟红细胞，为进一步探寻调控hESCs向成体造血分化的关键因子奠定了基础。本研究选择调控造血至关重要转录因子GATA-1和GATA-2，通过构建慢病毒介导的条件诱导式GATA-1和GATA-2基因沉默的hES/hiPS细胞系，探索调控GATA-1和GATA-2基因表达对hESC/hiPSC向成体造血分化的影响。这种条件诱导模式可在多西环素（Doxcycline，Dox）诱导下在特定分化阶段和分化谱系调控GATA-1/GATA-2表达（shRNA）。我们的研究可以精密地检测GATA-1/GATA-2对hESC/hiPSC向造血细胞定向分化的影响，从而为揭开人类初期造血的分子调控机制奠定基础。

The knowledge about the molecular ragulation of human early hematopoiesis is largely unknow, mostly because we lack a proper in vitro system to mimic the natural progressive course during the development from pluripotent stem cells to functionally mature blood cells. The esbablishment of human embryonic stem cells, and lately, induced pluripotent stem cells (hESC, hiPSC, respectively) greatly expanded our view to subtly analyze the cellular and molecular happenings during the early develoment of various tissues, including blood. Accumulated data, including ours, has achieved various functionally matured blood cells derived from hESC/hiPSCs, highlighting their ideal utilazation both as the research models and clinical applications. However, the hESC/hiPSC-derived hematipoietic cells were phenotpically and functionally immature, and their melacular controlling towards maturation being unclear. We here design a GATA-1/GATA-2 down-regulation system by using molecular targeting manipulation (shRNA)to transfect and establish hESC/hiPSC lines. Using this system, we can further analyze the early hematopoiesis in a subtle and timed way during the early differentiation of hematopoietic stem/progenitor cells from hESC/hiPSC. Our study may dedicate critical information about the molecular controls in human early hematopoiesis, especially the generation of definitive hematopoietic stem cells and their functionally matured progenies.