CTCF在单纯疱疹病毒Ⅱ型潜伏复发中的作用研究

单纯疱疹病毒Ⅱ型（HSV-2）引起的生殖器疱疹是常见性传播疾病，该病毒在体内长期潜伏并反复发作，临床上很难治愈，目前其复发机制尚不清楚。CTCF是存在于真核生物的多功能转录因子，能调控多种靶基因的表达，在HSV潜伏期间，有大量的CTCF结合在HSV潜伏相关转录体（LAT)周围，当外界非特异因素作用下，CTCF会从LAT周围的结合位点脱落，HSV病毒将被激活进行复制。HSV的潜伏复发机制与CTCF的关系值得我们进一步研究。本研究利用生物信息学预测HSV-2基因组中CTCF结合序列，通过体内外功能验证，确定CTCF在HSV-2抗凋亡及复发感染中的作用，为研究HSV-2病毒潜伏、复发机制提供了一种新的思路，为生殖器疱疹疾病抗复发治疗的新靶点提供坚实的理论基础。

Genital herpes (GH),a common sexually transmitted disease,is primary caused by Herpes Simplex Virus type 2(HSV-2),following primary infection at skin or mucosal surfaces,HSV-2 can stablishes a life-long latency in sensory neurons,there are no any anti-virus drugs,which can effectively control the occurrence and recrudescence of HSV-2,the machanism of HSV-2 latent infection and virus reactivation is poorly understood.CTCF is a multivalent eukaryotic transcription factor and can regulate multiple target genes,during the latency，CTCF positioned within the HSV-1 genome,specfically around the latency-associated transcript (LAT),when stimulated by external factors，CTCF fall from the binding site, the virus will be activated.So,the relation between CTCF and the recrudescence mechanism of HSV-2 is worth research.In this study, bioinformatic predictions will be used to find the possible CTCF binding site in HSV-2 genome and the role of CTCF in HSV-2 latent infection will be verified in vitro and in vivo.This research provides a new way for the study of HSV-2 latency and recrudescence and will provide adequate teoretical basis for the new target in the treatment of genital herpes disease.