前期的研究，我们证实了TESTIN基因在原发性胃癌中起抑制作用，并发现其主要通过启动子甲基化导致在多数胃癌组织中表达缺陷；过表达TESTIN可抑制胃癌细胞增殖和克隆形成，抑制胃癌细胞由G1进入S期，能显著抑制胃癌细胞侵袭和迁移；且能显著抑制胃癌细胞在裸鼠体内生长。结合文献分析，我们发现TESTIN与促进肿瘤细胞迁移和侵袭的基因密切关联，推测TESTIN可能在抑制胃癌细胞转移中发挥重要作用，因此，本课题拟开展TESTIN抑制胃癌细胞转移分子机制研究，主要包括检测与TESTIN相互作用的基因或蛋白质；探讨其可能参与的主要信号转导通路；检测与TESTIN相互作用的基因或者蛋白质在胃癌细胞的侵袭和迁移过程中的变化；利用小鼠体内胃癌引起的肺转移模型，检测TESTIN基因对胃癌细胞转移能力的影响；临床资料分析TESTIN与胃癌转移相关参数的关系等，初步阐明TESTIN基因抑制胃癌细胞转移的分子机理。

Our previous researches have confirmed the inhibitory effect of TESTIN in primary gastric carcinoma, and found that promoter methylation leaded to defected expression of TESTIN in most gastric carcinoma tissues. Overexpression of TESTIN gene can inhibit gastric cancer cell proliferation and colony formation, as well as inhibit the cell cycle from G1 into S phase. This gene can significantly inhibit the migration and invasion of gastric cancer cells in vitro. It could also significantly inhibit the tumorigenicity in nude mice. According to the literatures, we hypothesized that TESTIN gene may play an important role in inhibiting the metastasis of gastric carcinoma cells. Therefore in this project, we will study the molecular mechanisms of TESTIN on the inhibition of gastric cancer cell metastasis, including the detection of genes or proteins which could interact with TESTIN gene; the main signal transduction pathway may be involved in the process; the detection of TESTIN gene and its interacting gene or protein changes in the invasion and the migration process; using a mouse lung metastasis model to study the influence of TESTIN gene on metastasis of gastric cancer cells; analysis the relationship between the TESTIN and gastric cancer metastasis related parameters. We will prelimilary elucidate the molecular mechanisms of TESTIN gene on the inhibition of gastric cancer cell metastasis.