绵羊肺腺瘤病毒（JSRV）的囊膜蛋白(Env)在诱导肺上皮细胞癌变过程中起重要作用。为了探索Env与其受体结合后诱导肺上皮细胞癌变的信号转导机制，本课题拟在以往的研究基础上，制备具有感染性的JSRV囊膜假病毒，之后构建体外Env致肺腺癌细胞模型，检测病毒感染易感细胞后，细胞信号转导通路上的磷酯酰肌醇3-激酶、丝裂原活化蛋白激酶、端粒酶的表达。进一步回答JSRV的囊膜蛋白是否与受体相互作用而引起靶细胞的癌变？靶细胞信号转导通路上的关键酶的表达变化有没有规律可寻。在此基础上构建JSRV囊膜假病毒的小鼠感染模型和绵羊感染模型，在动物体内进一步分析Env致瘤的信号传导机制，进而分析该机制与肿瘤发生间的关联性。本课题为深入研究肿瘤病毒的分子致病机制奠定基础，同时为通过阻断传导通路而构建的变异型占位蛋白的研究提供前期理论基础。

Jaagsiekte retrovirus (JSRV) envelope protein (Env) is important cancer-causing protein in lung epithelial cells. In order to explore the mechanisms of signal transduction for the lung epithelial cells canceration induced by Env binding with its receptor, and on the basis of previous studies, this task will prepare JSRV envelope pseudovirus which has infectious, and then constructed Env induced pulmonary adenocarcinoma cell model in vitro. testing expression changes of phosphatidylinositol 3 - kinase, mitogen-activated protein kinases and telomerase in cell signal transduction pathways After virus infection of susceptible cells. Further answer whether JSRV envelope protein is the interaction with its receptor, and then Cancer-caused target cells? Expression of a key enzyme in the target cell signal transduction pathways have any rules can be found. On this basis, to build the infection model in mice and in sheep used the JSRV envelope pseudovirus, further analysis the signal transduction mechanisms of Env oncogenic in animals, and then analyzed the correlation between this mechanism and tumorigenesis. This project will lay the foundation for in-depth studies of the molecular pathogenesis by infect tumor viruses, as well as to provide an initial theoretical foundation to build the variant placeholder protein by blocking the signal transduction pathway.