microRNA-221和microRNA-125b在ALV-J致瘤过程中作用机制研究

J亚群禽白血病（ALV-J）作为家禽重要的病毒性肿瘤病，对我国养鸡业危害严重，致瘤机制的研究对该病的防控具有重要的理论指导意义。ALV-J诱导肿瘤产生受多种因素调控，有来自病毒自身的、也有来源于宿主细胞的。microRNA作为一种重要的调控分子参与了多种基因的转录后调控，microRNA在肿瘤细胞浸润、肿瘤血管形成过程中起着极为重要的作用。我们和他人的研究均证明，ALV-J感染的鸡肝脏肿瘤组织中，gga-miR-221和gga-miR-125b出现差异表达，这两个microRNAs分子在哺乳动物中已经作为与肿瘤密切相关的致瘤因子而被广泛研究。因而本项目将以宿主microRNAs分子gga-miR-125b和gga-miR-221为研究对象，首先确定其作用的靶分子，在此基础上深入研究其调控ALV-J致瘤的信号通路，从分子水平上揭示宿主microRNAs调控ALV-J的致瘤机制。

As the important chicken viral tumor, avian leukosis virus subgroup J (ALV-J) has induced enomous ecnomic loss to pourty in china. The study of tumorigenesis mechanism has important theoretical guiding significance to the prevention and control of the disease. The generation of tumor induced by ALV-J was modulated by a lot of factor, some from the virus itself, some from the host cell. As important regulatory molecules, microRNAs participate in a few gene's post-transcription regulation. microRNA played important role in infiltration of tumor cells and tumor angiogenesis. All study results of other researcher and our laboratory confirmed the dysregulation expression of gga-miR-221 and gga-miR-125b in liver tumor induced by ALV-J. Regarded as tumorigenic factors related to tumorigenesis closely, these two microRNAs molecules have been widely studied in mammals. The regulatory mechanisms of gga-miR-125b and gga-miR-221 to oncogenesis of ALV-J will be research in this project.